QUANTITATIVE DETERMINATION OF MONOSACCHARIDES OF LIQUID THYME CREEPING EXTRACT AND SELECTION OF ACCEPTANCE CRITERION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i2.12247Keywords:
creeping thyme, liquid extract, spectrophotometry, monosaccharides, acceptability criteriaAbstract
Introduction. In recent years, there has been a growing scientific interest in the study of polysaccharides in medicinal plant raw materials, which is due to their wide range of pharmacological action. Previous studies have shown that polysaccharides are present in extracts of creeping thyme (CT) obtained with water and solutions with low concentrations of alcohol, after hydrolysis of which monosaccharides have been obtained. Because, the development of a mucolytic agent based on a thick extract of emergency and essential oil of thyme involved, first of all, obtaining a liquid extract of emergency, which was obtained by the selected technology, which allowed to preserve and reproduce the analyzed BAS herbs. Therefore, in order to standardize the liquid extract of the state of emergency, it is important to determine not only their qualitative composition, but also the quantitative content, which will allow to choose the appropriate quality indicators and offer acceptability criteria.
The aim of the study – quantitative determination of monosaccharides in liquid extract of СT, selection of appropriate acceptance criteria.
Research Мethods. Spectrophotometer "Cary-50", liquid extract of creeping thyme, FSЕ: glucose (Fluka), 1 % solution of picric acid, 20 % solution of sodium carbonate, 20 %, 40 % solution of sodium hydroxide, dilute hydrochloric acid R.
Results and Discussion. Quantitative determination of monosaccharides in the liquid extract of СТ was performed by differential spectrophotometry in the visible part of the spectrum by reaction with picric acid. Their content varies within certain limits and is determined by the content in the raw material. Therefore, in order to standardize the liquid extract of СT, you can choose a quantitative quality criterion – the content of reducing monosaccharides, as well as to propose appropriate acceptance criteria – not less than 0.09 % in terms of glucose.
Conclusion. Quantitative determination of reducing monosaccharides in liquid extract of CT is carried out. As a result, their quantitative content was determined and eligibility criteria were proposed.
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