INFLUENCE OF ACUTE BLOOD LOSS COMPLICATED BY LIMB ISCHEMIA-REPERFUSION ON THE DYNAMICS OF GLUTATION ANTIPEROXIDASE INDICATORS IN THE SPLEEN AND ITS CORRECTION WITH CARBACETAM
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i2.12243Keywords:
acute blood loss, ischemia-limb reperfusion, spleen, reduced glutathione, glutathione peroxidase, carbacetamAbstract
Introduction. Disruption of the glutathione antiperoxidase system is one of the key mechanisms of acute blood loss and ischemic-reperfusion syndrome. The spleen plays an important role in adaptive-compensatory processes in the conditions of blood loss. However, the state of the glutathione antiperoxidase system in the spleen has not been studied enough. There are no data on the effectiveness under these conditions of carbacetam, which found a pronounced protective effect on the enzyme link of antioxidant protection of the spleen.
The aim of the study – to determine the dynamics of the glutathione antiperoxidase system of the spleen in conditions of acute blood loss complicated by ischemia-reperfusion of the limb and to evaluate the effectiveness of correction of the identified disorders by carbacetam.
Research Methods. 108 nonlinear male rats weighing 200–220 g were used in the experiments. All experiments were performed under thiopental-sodium anesthesia. In animals, limb ischemia-reperfusion and acute blood loss were simulated and these lesions were combined. In a separate group, the detected disorders were corrected with carbacetam. After 1 and 2 hours, as well as after 1, 7 and 14 days in the spleen of experimental animals was determined the content of reduced glutathione and the activity of glutathione peroxidase.
Results and Discussion. Modeling of acute blood loss, complicated by ischemia-reperfusion of the limb, in the spleen contributes to the greatest depletion of reduced glutathione and the activity of glutathione peroxidase, starting with 2 h of the experiment. After 1 day, the value of the studied indicators reached a minimum level and was statistically significantly less than in the conditions of modeling only acute blood loss. The use of carbacetam had a protective effect on the content of reduced glutathione in the spleen after 7 days of use, the activity of glutathione peroxidase – after 14 days, which puts carbacetam in a number of promising means of complex therapy of disorders caused by acute blood loss and ischemic-reperfusion syndrome.
Conclusions. Simulation of acute blood loss complicated by ischemia-reperfusion of the limb leads to a decrease in the content of reduced glutathione and glutathione peroxidase activity in the spleen with a maximum after 1 day of the experiment. The use of carbacetam promotes the protection of disorders of the content of reduced glutathione in the spleen after 7 days of use, glutathione peroxidase activity – after 14 days.
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