THE STATE OF LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN EXPERIMENTAL NEOPLASTIC INTOXICATION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i2.12234Keywords:
lipid peroxidation, antioxidant protection, neoplastic intoxicationAbstract
Introduction. The question of studying the imbalance between the processes of lipid peroxidation and antioxidant protection is relevant. This imbalance causes metabolic disorders, the formation of peroxides, ketones, aldehydes.
The aim of the study – to investigate the degree of activation of lipid peroxidation and the state of antioxidant protection in neoplastic intoxication syndrome. An important issue is the study of the imbalance between the processes of lipid peroxidation and antioxidant protection. This imbalance causes metabolic disorders, the formation of peroxides, ketones, aldehydes and it is the basis of the pathogeneses of endogenous intoxication. We investigated the degree of activation of lipid peroxidation and the state of antioxidant protection in the syndrome of neoplastic intoxication.
Research Methods. Pharmacological, morphological, methods of mathematical statistics. Experimental pharmacological studies were performed on white rats weighing 170–180 g. The studies were performed in the Central Research Laboratory of I. Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine (certificate No. 023/13 of May 05, 2020) in accordance with the methodological recommendations of the State Expert Center of the Ministry of Health of Ukraine. We studied the following blood biochemical parameters: the level of diene and triene conjugates, medium molecular weight peptides (at wavelengths of 254 and 280 nm), active products of thiobarbituric acid, superoxide dismutase and catalase activity. Rats included in the study were divided into 2 groups: 1 – control group and 2 – positive pathology; the study lasted 30 days. Modeling of pathological processes and withdrawal of animals from the experiment was carried out in compliance with the requirements of bioethics, rules of good laboratory practice and ethical standards of the “European Convention for the protection of vertebrate animals used for experimental or other scientific purposes”.
Results and Discussion. The study showed an increase in biochemical parameters of diene and triene conjugates, active products of thiobarbituric acid, which indicates the activation of the peroxidation process. When evaluating the indicators of superoxide dismutase, it was found that its level was fluctuating and by the end of the experiment decreased by 16.5 %. The level of catalase increased during the experiment. When studying the indicators of MMM254 and MMM280, their growth was revealed, which indicates an increase in the manifestations of endogenous intoxication.
Conclusion. With the syndrome of neoplastic intoxication in the blood there is a gradual excessive formation of products of free radical oxidation in combination with compensatory activation of enzyme units of the antioxidant system.
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