DEVELOPMENT OF MONOSACCHARIDES IDENTIFICATION METHODS IN THYME CREEPING EXTRACTS

Abstract

Introduction. The priority of modern pharmacy is the search for and development of new drugs based on medicinal plant raw materials. Pharmaceutical development on the basis of a thick extract of creeping thyme, initially, involved obtaining a liquid extract of creeping thyme (CT), which was obtained by technology that allowed to preserve the quality of biologically active substances (BAS) of the corresponding MPS. Since, when standardizing the herb creeping thyme with identification markers of quality, among others, monosaccharides were selected, so it is appropriate to study the qualitative composition of these BAS in the studied extract, because they are characterized by a wide range of pharmacological action.

The aim of the study – to develop a method for the identification of monosaccharides in liquid extract after preliminary hydrolysis and to choose appropriate quality markers.

Research Мethods. Liquid extract of creeping thyme, standard samples: arabinose, galactose, rhamnose, fructose, glucose, xylose (Fluka), thin layer chromatography (TLC), chromatographic plates Silica gel F254 company "Merck", device for automatic sampling on the plate Linomat 5 CAM ”, chromatographic camera“ GAMAG ”, ­UV‑lamp.

Results and Discussion. Approaching the standardization of liquid extract of creeping thyme, we considered the possibility of identifying similar BAS raw materials, continuing the proposed approach: medicinal plant raw material – extracts – drug. The qualitative composition of monosaccharides was determined by TLC in a system of solvents: water P – acetonitrile P in the ratio (15:85). Different methods and conditions of chromatography were taken into account for the development of the method of qualitative determination of the analyzed BAS in the liquid extract of CT, as a result, the optimal system of solvents with good resolution was selected, which was used to identify monosaccharides in the studied extract.

Conclusions. A method for qualitative determination of monosaccharides in liquid extract of creeping thyme has been developed. As a result of this study, seven monosaccharides were identified, one of which remained unknown due to the lack of the required standard, and quality markers of the studied extract (presence of fructose and glucose) were proposed.