MARKERS OF LYSOSOMAL DAMAGE IN THE COMBINED ACTION OF Κ-CARRAGEENAN AND MONOSODIUM GLUTAMATE IN THE EXPERIMENT
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i2.12053Keywords:
carrageenan, monosodium glutamate, acid phosphatase, cathepsin B, blood, liver, lungsAbstract
Introduction. Research highlights unresolved issues in the study of carrageenans, including the relationship between the physicochemical properties of carrageenan, its effect on digestive proteolysis, colon microbiome, and inflammation; as well as its effects in different risk groups, which indicates the need for further research to determine its possible toxic effects in the case of increasing levels of carrageenan in the human diet, as well as a wide combination of different supplements.
The aim of the study – to evaluate the indicators of lysosomal damage (acid phosphatase and cathepsin B activity) in the tissues of rats under the combined action of κ-carrageenan and monosodium glutamate.
Research Methods. The study was carried out on 48 white nonlinear male rats, which were divided into 4 groups: control (intact animals), 1 – animals that were intragastrically injected with κ-carrageenan at a dose of 40 mg/kg for 1 month, 2 – animals, which were intragastrically injected sodium glutamate at a dose of 50 mg/kg for 1 month, 3 – animals that were intragastrically injected with carrageenan and sodium glutamate in the above doses. The activity of acid phosphatase and cathexin B in the blood, lungs and liver of rats was determined by standard methods.
Results and Discussion. Significantly higher acid phosphatase activity was found in 1 and 2 experimental groups in the serum, as well as in the liver and lungs of rats, in relation to control. It should be noted the highest values of acid phosphatase activity in animals of the experimental group 3, which were probably higher in the serum (by 90.20 %), as well as in the liver (by 69.60 %) and lungs (by 82.20 %) of rats, relative to control. Significantly higher activity of cathepsin B in experimental groups 1 and 2 in the serum, as well as in the liver and lungs of rats, relative to control. It should be noted the highest values of cathepsin B activity in animals of the experimental group 3, which were probably higher in the serum (97.70 %), as well as in the liver (52.10 %) and lungs (83.10 %) of rats, regarding control.
Conclusions. In the combined action of solutions of κ-carrageenan and monosodium glutamate, the activity of acid phosphatase and cathepsin B in serum (by 90.2 % and 97.7 %), liver (by 69.6 % and 52.1 %) and lungs is likely (by 82.2 % and 83.1 %) of rats increase relative to control, which is also statistically significantly higher against the values of these indicators with separate use of κ-carrageenan, as well as monosodium glutamate.
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