SPECIFIC FEATURES OF PROTEINS OXIDATIVE MODIFICATION IN THE COMBINED ACTION OF FOOD ADDITIVES
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.i4.11602Keywords:
food additives, oxidative modification of proteins, blood, liver, lungs, effectAbstract
Introduction. A decrease in the life expectancy of the population of Ukraine, a sharp decrease in the quality of life and the health index of the nation with a significant violation of the nutritional status predetermines the need to raise issues of food quality, including the uncontrolled use of food additives, which determines the relevance of this study.
The aim of the study – to evaluate the indicators of oxidative modification of proteins in the tissues of the rat body when using a solution of κ-carrageenan, sodium glutamate and their combined action.
Research Methods. The study was carried out on 48 white nonlinear male rats, which were divided into 4 groups: 1 – control (intact animals), 2 – animals that were intragastrically injected with κ-carrageenan at a dose of 40 mg/kg for 1 month, 3 – animals, which intragastrically injected sodium glutamate at a dose of 50 mg/kg for 1 month, 4 – animals that were intragastrically injected with carrageenan and sodium glutamate in the above doses. To assess the spontaneous oxidative modification of proteins (OMP) spectrophotometrically in the ultraviolet part of the spectrum at a wavelength of 370 nm, neutral ketone dinitrophenylhydrazones (CDNPH) and in the visible light region – 430 nm, aldehyde dinitrophenylhydrazones of the basic nature (ADNPH) were determined. To assess the stimulated OMP 0.1 ml of prepared ex tempore 410-3 M FeSO4, 110-3 M EDTA, 310-4 M H2O2 was previously added to the experimental and control samples.
Results and Discussion. It was found that the proportion of primary (ADNPH) and secondary (CDNPH) markers in the blood serum did not practically differ from the ratio in the control group. In the lung tissues, when sodium glutamate was administered to experimental animals, the ratio of ADNPG to CDNPG changed towards an increase in the proportion of secondary markers of oxidative stress, while in other study groups their ratio practically did not differ from the control. In liver tissues, the proportion of primary and secondary markers in the experimental groups did not practically differ from the ratio in the control group. Analysis of the ratio of primary and secondary markers of oxidative modification of proteins indicates an increase in markers of late destruction in lung tissues with the use of sodium glutamate. The reduced proportion of secondary markers in the blood serum upon administration of carrageenan in relation to the group of animals that were injected with sodium glutamate may indicate a faster utilization of the altered proteins, the so-called secondary antioxidant effect.
Conclusions. In the combined action of solutions of κ-carrageenan and sodium glutamate, spontaneous oxidative modification of proteins significantly increases relative to the control and individual action of food additives, which is accompanied by depletion of reserve-adaptive potential in the blood, lungs and liver, respectively, by 33.4 %, 32.9 % and 24.0 %.
References
Kukk, M., & Torres, D. (2020). Risk assessment related to food additives and food processing-derived chemical contaminants exposure for the Portuguese population. University of Porto, Faculty of Nutrition and Food Sciences. EFSA Journal, 18 (1), 10 .
(2008). Regulation (EC) No 1333/2008 of the European Parliament and of the Council of 16 December 2008 on food additives. European Union.
Smolyar, V.I. (2005). Toksychni efekty kharchovykh dobavok [Toxic effects of food additives]. Problemy kharchuvannia – Nutrition Problems, 1, 5-15 [in Ukrainian].
https://zakononline.com.ua/documents/show/ 198223___570498
Pro Natsionalnyi plan dii na 2013 rik shchodo vprovadzhennia Prohramy ekonomichnykh reform na 2010-2014 roky “Zamozhne suspilstvo konkurentnospromozhna ekonomika, efektyvna derzhava” [On the National Action Plan for 2013 on the implementation of the Program of economic reforms for 2010-2014 "Wealthy society, competitive economy, efficient state"]. Ukaz Prezydenta Ukrainy – Decree of the President of Ukraine, March 12, 2013 No. 128 [in Ukrainian].
(2010). Commission Regulation (EU) No 257/2010 of 25.03.2010 setting up a programme for the re-evaluation of approved food additives in accordance with Regulation (EC) No 1333/2008 of the European Parliament and of the Council on food additives. European Union.
Koshkalda, I.V. (2017). Aktualni pytannia prodovolchoho zabezpechennia [Current issues of food supply]. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia “Ekonomika i menedzhment” – Bulletin of Sumy National Agrarian University. Series "Economics and Management", 4 (71), 207-212 [in Ukrainian].
Ivanenko, T.O., Korobchanskyy, V.O., Hubina-Vakulyk, H.I., Horbach, T.V., & Kolusova, N.H. Sposib modeliuvania khronichnoho hastroenterokolitu [Method of modeling chronic gastroenterocolitis]. Pat. 97322 Ukraine, MPK G09B 23/28. № a201014510, stat. 06.12.2010, publish. 25.01.2012, 2 [in Ukrainain].
Moyana, T.N., & Lalonde, J.M. (1990). Carrageenan-induced intestinal injury in the rat – a model for inflammatory bowel disease. Ann. Clin. Lab. Sci., 20, 6, 420-426.
Falaleeva, T.M., Samonina, H.E., & Berehovaya, T.V. (2010). Vliyanie gliprolinov na strukturno-funktsionalnoe sostoyanie slizistoy obolochki zheludka i massy tella krys v usloviyakh dlitelnogo vvedeniya glutamata natriya [Influence of glyprolines on the structural and functional state of the gastric mucosa and body weight of rats under conditions of prolonged administration of sodium glutamate]. Fizika zhivogo – Physics of the Living, 18,1, 154-159 [in Russian].
Dosvyadchynska, M.R., & Antoniak, H.L. (2012). Peroksydne okysnennia lipidiv ta aktyvnyst antyoksydantnykh enzymiv u klitynakh lehen shchuriv za shchodobovoho vvedennia aflatoksynu B1 [Lipid peroxidation and activity of antioxidant enzymes in rat lung cells with daily administration of aflatoxin B1]. Biolohiia tvaryn – Animal Biology, 14, 1-2, 108-112 [in Ukrainian].
Dubinina, E.E. (2000). Okislitelnaya modifikatsiya belkov plazmy krovi bolnykh psikhicheskimi rasstroystvami (depresiya, depersonalizatsiya) [Oxidative modification of blood plasma proteins in patients with mental disorders (depression, depersonalization)]. Voprosy meditsinskoy khimii – Issues of Medical Chemistry, 46, 4, 398-409 [in Russian].
Lowery, O.H., Rosebroughh, N.I., Farr, A.L., & Randall, R.I. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193 (1), 265-275.
Jones, L.A., & Holmes, R.B. (1956). Spectrophotometric studies of some 2,4-dinitrophenylhydrazones. Analytical Chemistry, 28, 2, 191-198.
Shevelkova, A.A., & Vjushina, A.V. (2012). Okislitelnaya modifikatsiya belkov i soderzhanie tiolov v krovi pri fiziologicheski protekayushchey beremennosti [Oxidative modification of proteins and the content of thiols in the blood during physiological pregnancy]. Zhurnal akusherstva i zhenskikh bolezney – Journal of Obstetrics and Women's Diseases, 1 (4), 109-112 [in Russian].
Gubskij, Ju.I. (2005). Toksikologicheskie posledstviya okislitelnoy modifikatsii belkov pri razlichnykh patologicheskikh sostoyaniyakh [Toxicological consequences of oxidative modification of proteins in various pathological conditions]. Sovremennye problemy toksikologii – Modern Problems of Toxicology, 8, 3, 20-27 [in Russian].
Dubinina, E.E. (2006). Produkty metabolizma kisloroda v funktsionalnoy aktivnosti kletok (zhizn i smert, sozidanie i razrushenie). Fiziologicheskie i kliniko-biohimicheskie aspekty [Oxygen metabolism products in the functional activity of cells (life and death, creation and destruction). Physiological and clinical-biochemical aspects]. St. Petersburg: Meditsinskaya pressa [in Russian].
Vedunova, M.V., Sazanov, A.I., Kontorshhikova, K.N. (2010). Vliyanie nizkikh terapevticheskikh doz ozona na uroven okislstelnoy modifikatsii belkov [Effect of low therapeutic ozone doses on the level of oxidative modification of proteins]. Vestnik Nizhegorodskogo universiteta im. N.I. Lobachevskogo – Bulletin of the Nizhny Novgorod University by N.I. Lobachevsky, 2, 504-507 [in Russian].
Ergin, V., Hariry, R.E., & Karasu, Ç. (2013). Carbonyl stress in aging process: Role of vitamins and phytochemicals as redox regulators. Aging and Disease, 4, 5, 279-294.
Mjalyuk, O.P. (2015). Stan vilnoradykalnoho okysnennia u tkanynakh pechinky eksperymentalnykh shchuriv pry alimentarnomu ozhyrinni [The state of free radical oxidation in the liver tissues of experimental rats in alimentary obesity. Bulletin of problems of biology and medicine]. Visnyk problem biolohii i medytsyny – Bulletin of Problems of biology and Medicine, 4, 1 (124), 120-123 [in Ukrainian].
Krynytska, I., Marushchak, M., Svan, O., Akimova, V., Mazur, L., & Habor, H. The indices of endogenous intoxication in rats with carrageenan solution consumption. Georgian Medical News (279), 196-200.
Krynytska, I., Marushchak, M., & Rutska, A. (2019). Gender-specific differences of oxidative processes in the population of circulating neutrophils of rats in a setting of prolonged administration of monosodium glutamate. Romanian Journal of Diabetes Nutrition and Metabolic Diseases, 26, 2, 119-127.
Zyn, A., Holovchak, N., & Sanhurskyi, D. (2012). Okysna modyfikatsiia bilkiv u zarodkakh viuna MISGURNUS FOSSІLIS L. Uprodovzh embriohezu za dii hipokhlorytu natriiu [Oxidative modification of proteins in embryos of MISGURNUS FOSSILIS L. during embryogenesis under the action of sodium hypochlorite]. Visnyk Lvivskoho universytetu. Seriia biolohichna – Bulletin of Lviv University. Biological Series, 61, 11-19 [in Ukrainian].
Marushchak, M. (2013). Rol aktyvnyh form kysniu u rozvytku i prohresuvanni hostroho urazhennia lehen v eksperymenti [The role of reactive oxygen species in the development and progression of acute lung injury in the experiment]. Medychna khimiia – Medical Chemistry, 14, 1, 104-108 [in Ukrainian].
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Medical and Clinical Chemistry
This work is licensed under a Creative Commons Attribution 4.0 International License.
