SEASONAL VARIATION IN THE ASCORBIC AND ORGANIC ACIDS CONTENT IN SHOOTS OF HIGHBUSH BLUEBERRY CULTIVARS DURING VEGETATION STAGES
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i2.11355Keywords:
Vaccinium corymbosum L., Bluejay, Bluecrop, Elliott, ascorbic acid, organic acidsAbstract
Introduction. Ascorbic acid (AA) and organic acids (OА) are synthesized in all plants, and are an important part of their metabolism, as well as an integral part of the metabolism of the human body. The study of the mechanisms of action of highbush blueberry (HB) extracts is preceded by the most detailed study of the composition, in particular AA and OA, the content of which may vary in different physiological phases of development.
The aim of the study – to determine the content of ascorbic and organic acids in the HB shoots of three varieties at different stages of their physiological development.
Research Methods. The material for the study was shoots of blueberry (Vaccinium corymbosum L.) varieties Bluejay, Bluecrop and Elliott. The AA content was determined spectrophotometrically; the content of OA was determined titrimetrically.
Results and Discussion. As a result of the analysis, the level of AA and OA was almost at parity with each other in investigated varieties of HB. The value of AA was recorded 42.61–83.62 mg·100 g-1 DW (the lowest is (42.61±1.828) in IV phase) in Bluejay; 49.02–102.5 mg·100 g-1 DW in Bluecrop; 70.87–98.04 mg·100 g-1 DW in Elliott. Changes OA contents were within 2.19–5.26 % in Bluejay; 3.12–7.83 % in Bluecrop, and 3.80–9.16 % in Elliott.
Conclusions. Varieties of highbush blueberries with different fruit ripening dates differ in the content of AA and OA in the shoots during the growing season. The shoots of V. corymbosum varieties Bluecrop. Bluejay, and Elliott are sufficiently high in AA and OA during physiological development, and can be used for further research.
References
Davey, M.W., Van Montagu, M., Inzé, D., Sanmartin, M., Kanellis, A., Smirnoff, N., et al. (2000) Plant L-ascorbic acid: chemistry, function, metabolism, bio_availability and effects of processing. J. Sci. Food Agric., 80, 825-860.
Smirnoff, N., & Wheeler, G.L. (2000) Ascorbic acid in plants: biosynthesis and function. Crit. Rev. Biochem. Mol. Biol., 35(4), 291-314. DOI: https://doi.org/10.1080/10409230008984166
Fenech, M., Amaya, I., Valpuesta, V., & Botella, M.A. (2019). Vitamin C content in fruits: biosynthesis and regulation. Front Plant Sci. Retrieved from: https://doi.org/10.3389/fpls.2018.02006. DOI: https://doi.org/10.3389/fpls.2018.02006
Linster, C.L., & Clarke, S.G. (2008) L-Ascorbate biosynthesis in higher plants: The role of VTC2. Trends in Plant Science, 13 (11), 567-573. DOI: https://doi.org/10.1016/j.tplants.2008.08.005
Pallanca, J.E., & Smirnoff, N. (2000). The control of ascorbic acid synthesis and turnover in pea seedlings. J. Exp. Bot., 51(345), 669-674. DOI: https://doi.org/10.1093/jexbot/51.345.669
Conklin, P.L. (2001) Recent advances in the role and biosynthesis of ascorbic acid in plants. Plant, Cell and Environment, 24 (4), 384-394. DOI: https://doi.org/10.1046/j.1365-3040.2001.00686.x
Noctor, G., Mhamdi, A., & Foyer, C.H. (2014). The roles of reactive oxygen metabolism in drought: not so cut and dried. Plant Physiol., 164, 1636-1648. DOI: https://doi.org/10.1104/pp.113.233478
Vorobets, N.M. (2003). Funktsionuvannia askorbatperoksydazy ta vmist askorbinovoi ta dehidroaskorbinovoi kyslot u prorostaiuchomu nasinni soniashnyku ta kvasoli pid diieiu riznykh doz svyntsiu [Functioning of ascorbate peroxidase and the content of ascorbic and dehydroascorbic acids in germinating sunflower and bean seeds under the action of different doses of lead]. Naukovyi visnyk Uzhhorodskoho natsionalnoho un-tu. Ser. biol. – Scientific Bul. Uzhhorod National Univ. Ser. Biol.,13, 53-56 [in Ukrainian].
Gallie, D.R. (2013). The role of l-ascorbic acid recycling in responding to environmental stress and in romoting plant growth. J. Exp. Bot., 64, 433-443. DOI: https://doi.org/10.1093/jxb/ers330
Broad, R.C., Bonneau, J.P., Hellens, R.P., & Johnson, A.A.T. (2020) Manipulation of ascorbiate biosynthetic, recycling and regulatory pathways for improved abiotic stress tolerance in plants. Int. J. Mol. Sci., 21 (5), 790.
Yabuta, Y., Mieda, T., Rapolu, M., Nakamura, A., Motoki, T., & Maruta, T., et al. (2007). Light regulation of ascorbate biosynthesis is dependent on the photosynthetic electron transport chain but independent of sugars in Arabidopsis. J. Exp. Bot., 58(10), 2661-2671. DOI: https://doi.org/10.1093/jxb/erm124
Del Bo, C., Riso, P., Campolo, J., Moller, P., Loft, S., Klimis-Zacas, D., et al. (2013). A single portion of blueberry (Vaccinium corymbosum L.) improves protection against DNA damage but not vascular function in healthy male volunteers. Nutr. Res., 33, 220-227. DOI: https://doi.org/10.1016/j.nutres.2012.12.009
Smirnoff, N. (2018). Ascorbic acid metabolism and functions: a comparison of plants and mammals. Free Radic. Biol. Med., 122, 116-129. DOI: https://doi.org/10.1016/j.freeradbiomed.2018.03.033
Saito, K., & Loewus, F.A. (1992). Conversion of D-glucosone to oxalic acid and L-(+)-tartaric acid in detached leaves of Pelargonium. Phytochemistry, 31, 3341-3344. DOI: https://doi.org/10.1016/0031-9422(92)83681-N
Lo´pez-Bucio, J., Nieto-Jacobo, M.F., Ramirez-Rodriguez, V., & Herrera-Estrella, L. (2001). Organic acid metabolism in plants: From adaptive physiology to transgenic varieties for cultivation in extreme soils. Plant Science, 160 (1), 1-13.
Macknight, R.C., Laing, W.A., Bulley, S.M., Broad, R.C., Johnson, A.A., & Hellens, R.P. (2017). Increasing ascorbate levels in crops to enhance human nutrition and plant abiotic stress tolerance. Curr. Opin. Biotechnol., 44, 153-160. DOI: https://doi.org/10.1016/j.copbio.2017.01.011
Du, J., Cullen, J.J., & Buettner, G.R. (2012). Ascorbic acid: chemistry, biology and the treatment of cancer. BBA, 1826, 443-457. DOI: https://doi.org/10.1016/j.bbcan.2012.06.003
Myllyharju, J. (2008). Prolyl 4-hydroxylases, key enzymes in the synthesis of collagen and regulation of the response to hypoxia, and their roles as treatment targets. Ann. Med., 40(6), 402-417. DOI: https://doi.org/10.1080/07853890801986594
Van Vlies, N., Wanders, R.J., & Vaz, F.M. (2006). Measurement of carnitine biosynthesis enzyme activities by tandem mass spectrometry: differences between the mouse and the rat. Anal. Biochem., 354 (1), 132-139. DOI: https://doi.org/10.1016/j.ab.2006.04.007
Ma, L., Sun, Z., Zeng, Y., Luo, M., & Yang, J. (2018). Molecular mechanism and nealth role of functional ingredients in blueberry for xhronic disease in human beings. Int. J. Mol. Sci., 19(9), E2785. DOI: https://doi.org/10.3390/ijms19092785
Linster, C.L., & Van Schaftingen, E. (2007). Vitamin C: biosynthesis, recycling and degradation in mammals. FEBS J., 274, 1-22. DOI: https://doi.org/10.1111/j.1742-4658.2006.05607.x
Liato, V., Hammami, R., & Aïder, M. (2017). Influence of electro-activated solutions of weak organic acid salts on microbial quality and overall appearance of blueberries during storage. Food Microbiol., 64, 56-64. DOI: https://doi.org/10.1016/j.fm.2016.12.010
Diaconeasa, Z. (2018). Time-dependent degradation of polyphenols from thermally-processed berries and their in vitro antiproliferative effects against melanoma. Molecules, 23 (10), 2534.
Yavorska, N., & Vorobets, N. (2019). The effect of variation of harvest season on water soluble BAS in shoots of Vaccinium corymbosum L. Book Abstracts. 4th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf. Albena resort, Bulgaria.
Vorobets, N., & Yavorska, N. (2019). Phytosynthetic pigments in shoots of Vaccinium corymbosum L. (cv. Elliott). Agrobiodiversity for Improving Nutrition, Health and Life Quality; Ed.: J.Brindza, S.Klymenko. Slovak Univ. of Agriculture in Nitra. ISBN 978-80-552-2108-3. Retrieved from: http: //doi.org/10.15414/agrobiodiversity.2019.2585-8246.093-100.
Hewitt, E.J., & Dickes, G.J. (1961). Spectrophotometric measurements on ascorbic acid and their use for the estimation of ascorbic acid and dehydroascorbic acid in plant tissue. The Biochem. J., 78 (2), 384-391. DOI: 10.1042/bj0780384. (2015). DOI: https://doi.org/10.1042/bj0780384
Derzhavna Farmakopeia Ukrainy: v 3 t. Derzhavne pidpryiemstvo “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv” [State Pharmacopeia of Ukraine: in 3 vol. / State Enterprise “Ukrainian Scientific Pharmacopoeial Centre of Medicinal drugs Quality]. Kharkiv: Derzhavne pidpryiemstvo “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv” [in Ukrainian].
Guarnieri, S., Riso, P., & Porrini, M. (2007). Orange juice vs vitamin C: Effect on hydrogen peroxide-induced DNA damage in mononuclear blood cells. Br. J. Nutr., 97 (4), 639-643. DOI: https://doi.org/10.1017/S0007114507657948
Beck, K., Conlon, C.A., Kruger, R., Coad, J., & Stonehouse, W. (2011). Gold kiwifruit consumed with an iron-fortified breakfast cereal meal improves iron status in women with low iron stores: a 16-week randomised controlled trial. Br. J. Nutr., 105 (1),101-109. DOI: https://doi.org/10.1017/S0007114510003144
Tugnoli, B., Giovagnoni, G., Piva, A., & Grilli, E. (2020). From acidifiers to intestinal health enhancers: How organic acids can improve growth efficiency of pigs. Animals., 10 (1), 134. Retrieved from: https://doi.org/10.3390/ani10010134 DOI: https://doi.org/10.3390/ani10010134
