DETERMINATION OF ARBUTIN IN THE PYRUS COMMUNIS LEAVES OF THE LYMONKA, LISOVA KRASUNIA AND NOIABRSKA VARIETIES
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i1.11062Keywords:
Pyrus communis, arbutin, qualitative and quantitative analysis, chromatography, spectrometryAbstract
Introduction. Cystitis is one of the quite common nosological forms of infectious-inflammatory diseases of the urinary tract. Prevalence of acute cystitis in Ukraine is 314 per 100 thousand population, chronic cystitis – 135 per 100 thousand population. For the treatment of this disease successfully used medicinal herbal raw materials containing arbutin and hydroquinone, in particular raw pear.
The aim of the study – to identify and quantify arbutin in the pear of ordinary leaves of the Lymonka, Lisova krasunia and Noiabrska varieties.
Research Methods. The subject of the study were pears of ordinary leaves Lymonka, Lisova krasunia and Noiabrska varieties. Detection of arbutin was carried out by chemical reactions and chromatography in a thin layer of sorbent. The quantitative content was determined by spectrophotometric method at a wavelength of 455 nm by the method of State Pharmacopoeia of Ukraine.
Results and Discussion. As a result of the chemical reactions and chromatographic analysis, the presence of arbutin was established in the pear of the ordinary leaves of the varieties studied. The content of arbutin, determined by the spectrophotometric method, in the pear of ordinary leaves of the Lymonka, Lisova krasunia and Noiabrska varieties was 1.07 %, 3.32 % and 3.01 %, respectively.
Conclusions. The pear of the ordinary leaves of the Lymonka, Lisova krasunia and Noiabrska varieties established the presence and determined the content of arbutin, which amounted to 1.07 %, 3.32 % and 3.01 %, respectively. The obtained experimental data will be used for standardization of pear of ordinary leaves, development of quality control methods for the raw material under study, as well as preparation of pharmacologically active substances based on it.
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