GENDER ASPECTS OF CARDIOPROTECTIVE EFFICIENCY OF MELATONIN AT THE DEVELOPMENT OF ADRENALINE-INDUCED MYOCARDIAL NECROSIS ON THE PERMANENT LIGHT BACKGROUND
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i1.11057Keywords:
permanent illumination, myocardial necrosis, free radical oxidation, sex differenceAbstract
Introduction. Circadian rhythm disturbance is a risk factor for ischemic heart disease, which is more common in men. Disturbance of the lighting regime (its excess) leads to deficit of endogenous melatonin and its antioxidant properties. That allows one to predict a decrease in the negative effect of stress or catecholamines on the heart at permanent light for using exogenous melatonin. To date, the gender difference in cardioprotective properties of melatonin in conditions of impaired activity of the pineal gland is not well understood.
The aim of the study – investigation of the cardioprotective effect of exogenous melatonin in the development of myocardial necrosis on the background of permanent light depending on sex.
Research Methods. Adrenalin-induced myocardium necrosis (MN) caused by adrenalin (0.5 mg/kg intramuscular) on a background of permanent illumination (10 days, illumination 500 lux/24 hours). Percentage of necrotized cardiomyocytes (PNC), lipid peroxidation (LPO) and antioxidant system (AOS) activity were determined in the myocardium. Melatonin (5 mg/kg, intraperitoneal) was administered 1 hour before adrenaline. Animals of controle group were in condition of day/night balance (12 h illumination 500 Lux and 12 h illumination 0.5-1 Lux).
Results and Discussion. Development of MN at melatonin use was characterized by less PNC, less activity of LPO, higher activity of superoxide dismutase and glutathione peroxidase. The better balance of prooxidant-antioxidant processes was evidenced by the value of the antioxidant-prooxidant index, especially in females. This pattern demonstrated the positive effect of exogenous melatonin and indirectly confirmed its deficiency under conditions of permanent illumination.
Conclusion. Melatonin has a positive effect on the development of adrenaline-induced myocardial necrosis on a background of permanent illumination, which is confirmed by less percentage of necrotized cardiomyocyte, less activity of lipid peroxidation, less depression of the antioxidant system. Female rats are more sensitive to the positive effect of exogenous melatonin compared to males.
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