ACTIVITY OF CATHEPSINS B, L AND H IN BLOOD PLASMA IN PATIENTS WITH CHRONIC DIFFUSE LIVER DISEASES
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i1.11052Keywords:
cathepsin B, cathepsin H, cathepsin L, α1-antitrypsin, α2-macroglobulin, chronic diffuse liver diseases, serological fibrosis biomarkersAbstract
Introduction. Plasma markers are widely used along with trepanobiopsy to diagnose the histological stages of chronic diffuse liver diseases.
The aim of the study – to determine the activity of cysteine cathepsins B, L, H and the content of proteolysis inhibitors α1-antitrinsin and α2-macroglobulin in the blood plasma of patients with chronic diffuse liver diseases of non-viral etiology.
Research Methods. The object of research is the blood plasma of patients with chronic diffuse liver diseases (n=51) aged 28–60 years hospitalized in the Department of Liver and Pancreas Diseases of the Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine. The control group consisted of healthy volunteers (n = 15) aged from 25 to 52 years. The activity of cathepsins B, L and H were determined relatively to N-α-benzoyl-DL-arginine-4-nitroanilide hydrochloride, to azocasein and to oxytocin, respectively. The solution of N-α-benzoyl-DL-arginine-4-nitroanilide hydrochloride was used as a substrate for determination the content of inhibitors in human blood plasma.
Results and Discussion. Compared with the group of practically healthy donors, statistically significant differences were recorded in: patients with steatohepatitis, the activity of cathepsin B increases by 26.7 %, and the level of α2-macroglobulin decreases by 30.7 %; in patients with chronic viral hepatitis with transition to cirrhosis, the activity of cathepsin B increases by 43.8 %, the activity level of cathepsin H decreases by 35 %, and the content of α2-macroglobulin, on the contrary, increases by 71.5 %; in the group of patients with steatohepatosis, the activity of cathepsin L and H decreases by 22.1 % and 25 %, respectively, and the concentration of α1-antitrypsin increases by 19.3 %.
Conclusions. Determination of the content of inhibitors in conjunction with the activity of cysteine cathepsin in blood plasma can be proposed as non-invasive markers for chronic diffuse liver diseases of non-viral etiology.
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