EFFECT OF MODULATORS OF NITRIC OXIDE SYNTHESIS ON HEMOSTASIS INDICATORS IN EXPERIMENTAL ANTIPHOSPHOLIPID SYNDROME

Abstract

Introduction. Antiphospholipid syndrome (APS) is an autoimmune disease characterized by arterial and venous thrombosis, thrombocytopenia. APS causes habitual miscarriage in 27–42 % of cases.

The aim of the study – to evaluate the effect of L-arginine and aminoguanidine on the blood clotting system and platelet level in experimental antiphospholipid syndrome before and in pregnancy.

Research Methods. The APS was modeled on BALB/c female mice. L-arginine (25 mg/kg) and aminoguanidine (10 mg/kg) were used for its correction. Platelet counts, fibrinogen concentration and coagulation rates were determined.

Results and Discussion. According to the research results, a decrease in platelet count, an increase in fibrinogen concentration, a shortening of activated partial thromboplastin time (aPTT), a reduction in prothrombin time, an increase of prothrombin correlation index was established in the animals with APS before pregnancy and on the 18^th day of pregnancy compare to the control groups. With introduction of L-arginine into the mice with APS before pregnancy and on the 18^th day of pregnancy an increase in platelet counts and a decrease in fibrinogen concentration were evidenced relative to the animals with APS. At the same time, in the APS animals the aPTT was further shortened; the pregnant APS mice experienced prolonged aPTT and increased international normalized ratios. With introduction of aminoguanidine into the APS mice, a further shortening of aPTT, a decrease in prothrombin time and international normalized ratio relative to the intact animals were proved. The increase of prothrombin index with introduction of aminoguanidine into the animals with APS before pregnancy and during pregnancy was established.

Conclusions. The tendency to hypercoagulation and the risk of thrombosis in females with APS have been established. It is one of the leading pathobiochemical aspects of pregnancy miscarriage in this disorder. L-arginine contributes to normal hemocoagulation and regulation of platelet counts; aminoguanidine leads to a worsening of the studied parameters of the coagulogram in APS before and during pregnancy.