GENDER DIFFERENCE OF FREE-RADICAL PROCESSES IN THE MYOCARDIUM AT ADRENALINE DAMAGE ON THE BACKGROUND OF LIGHT DEPRIVATION
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i4.10821Keywords:
light deprivation, myocardium necrosis, lipoperoxidation, gender differenceAbstract
Introduction. Coronary heart disease is the leading cause of death and is more common in men. Stress is prevalent risk factors of heart damage. The balance of the oxidant/antioxidant systems is important marker of metabolism disorder im myocardium at stress. It is proved that melatonin as antioxidant reduces adrenalin-induced myocardium necrosis. But, the gender difference of cardioprotective effect of melatonin in conditions of impaired pineal gland has not been sufficiently studied.
The aim of the study – to explore gender differences of lipid peroxidation activity in the myocardium at adrenalin-induced damage on the background of light deprivation.
Research Methods. In the myocardium of the ventricles of male and female rats, the activity of lipid peroxidation (LPO) and antioxidant system (AOS) was determined in case of adrenaline damage. Myocardial necrosis was simulated by introducing adrenaline (0.5 mg/kg) into the muscle against the background of light deprivation (10 days of complete darkness, lighting 0.5–1 LX). The control group consisted of animals under conditions of a preserved regime of day/night cycle change (12 hours of lighting 500 LX/12 hours of darkness 0.5–1 LX).
Results and Discussion. The development of the necrotic process in the rat myocardium on the background of ten-day light deprivation was accompanied by less activation of LPO compared with the indices of the animals of the control group. This was confirmed by a lower content of LPO products in the ventricular myocardium. AOS activity under such conditions was greater than in animals of the control group, especially in females. This regularity testified to the positive effect of preliminary ten-day light deprivation on the development of the necrotic process in the myocardium, which can be explained by enhanced synthesis of melatonin, which is an antioxidant, and was also characteristic of rats of both sexes, but the cardioprotective effect of light deprivation was more significant in females.
Conclusion. Light deprivation helps protect the heart from adrenaline damage, as evidenced by the lower activity of lipid peroxidation and the greater activity of enzymes of the antioxidant system, especially in females.
References
Baraboi, V.A., & Reznikov, O.H. (2013). Fiziolohiia, biokhimiia i psykholohiia stresu [Physiology, biochemistry and psychology of stress]. Kyiv [in Ukrainian].
Boland, T.A., Lee, V.H., & Bleck, T.P. (2015.) Stress-induced cardiomyopathy. Crit. Care Med., 43 (3), 686-693. DOI: https://doi.org/10.1097/CCM.0000000000000851
Prasad, A., Lerman, A., & Rihal, C.S. (2008). Apical ballooning syndrome (So-Tsubo or stress cardiomyopathy): a mimic of acute myocardial infarction. Am. Heart J.,155 (3),408-417. DOI: https://doi.org/10.1016/j.ahj.2007.11.008
Wang, A.R., Dean, S.A., Grebe, S.K., & Hood, I.C. (2016). Fatal catecholamine-induced cardiotoxicity associated with pheochromocytoma: Report of a postpartum case and literature review. Acad. Forensic Pathol., 6 (2), 315-324. DOI: https://doi.org/10.23907/2016.032
Khara, M.R., Shkumbatiuk, O.V., & Kuchyrka, L.I. (2014). Kharakterystyka stupenia poshkodzhennia miokarda adrenalinom u shchuriv riznoi stati zalezhno vid tryvalosti sposterezhennia ta rivnia statevykh hormoniv [Characteristics of the degree of myocardial damage by adrenaline in different sex rats, depending on the duration of observation and the level of sex hormones]. Visnyk morfolohii – Bulletin of Morphology, 2 (20), 372-375 [in Ukrainian].
Kazimirko, V.K., Ivanitskaya, L.N., Kutovoy, V.V., Dubkova, A.G., & Silantyeva, T.S. (2014). Perekisnoe okislenie lipidov: protivorechiya problem [Lipid peroxidation: contradictions of the problem]. Ukr. revmatol. zhurn. – Ukrainian Rheumatology Journal, 3 (57), 13-17 [in Russian].
Jiki, Z., Lecour, S., & Nduhirabandi, F. (2018) Cardiovascular benefits of dietary melatonin: A myth or a reality? Front Physiol., 9, 528.
Russel J. Reiter, Dun Xian Tan, Sergio Rosales-Corral, Annia Galano, Xin Jia Zhou, & Bing Xu. (2018). Mitochondria: Central organelles for melatonin’s antioxidant and anti-aging actions. Molecules, 23 (2), 509.
Glen Pyle, W., & Tami, A. Martino. (2018). Circadian rhythms influence cardiovascular disease differently in males and females: role of sex and gender. Current Opinion in Physiology, 5, 30-37. DOI: https://doi.org/10.1016/j.cophys.2018.05.003
Khara, M.R., & Shkumbatyuk, O.V. (2014). Statevi vidminnosti metabolichnykh zmin v miokardi pry rozvytku nekrotychnoho protsesu na tli melatoninu [Gender differences of metabolic changes in myocardium in the development of necrotic process on the background of melatonin]. Aktualnye problem transportnoy meditsiny – Actual Problems of Transport Medicine, 2 (36-II), 47-52 [in Ukrainian].
Gavrillov, V.B., & Mishorudnaya, M.I. (1983). Spektrophotometricheskoye opredelenie soderzhaniya hydroperekisey lipidov v plazme krovi [Spectrophotometric determination of lipid hydroperoxide content in blood plasma]. Lab. delo – Laboratory Case, 3, 33-35 [in Russian].
Andreeva, L.I., Kozhemyakin, L.A., & Kishkun, A.A. (1988). Modifikatsiya metoda opredeleniya perekisey lipidov v teste s tiobarbiturovoy kislotoy [Modification of the method of determination of lipid peroxides in the test with thiobarbituric acid]. Lab. delo – Laboratory Case, 11, 41-43 [in Russian].
Chevari, S., Chaba, I., & Sokey, Y. (1985). Rol superoxiddismutazy v okislitelnykh protsessakh kletki i metod opredeleniya ee v biologicheskom materiale [The role of superoxide dismutase in the oxidative processes of the cell and the method of its determination in biological material]. Lab. delo – Laboratory Case, 11, 678-681 [in Russian].
Korolyuk, M.A., Ivanova, L.I., & Mayorov I.G. (1988). Metod opredeleniya aktivnosti katalazy [The method of determining the activity of catalase]. Lab. delo – Laboratory Case, 1, 16-19. [in Russian].
Verevkina, N.V., Tochilkin, A.I., & Popova, N.A. (1977). Kolorimetricheskiy metod opredelenia SH-grupp i SS-sviazei pri pomoschi 5,5-ditiobis (2-nitrobenzonovoi) kisloty [A colorimetric method for the determination of SH groups and SS bonds in proteins by 5.5-dithiobis (2-nitrobenzoic) acid]. Modern methods in biochemistry. Moscow: Meditsina [in Russian].
Kruhlykova, H.O., & Stutman, Ts.M. (1976). Hlutationperoxydasna ta hlutationreduktazna aktyvnist pechinky shchuriv pislia vvedennia selenitu natriu [Glutathione peroxidase and glutathione reductase activity of rat liver after administration of sodium selenite]. Ukr. biokhim. zhurn. – Ukrainian Biochemical Journal, 48 (2), 227-233 [in Ukrainian].
Manchester, L.C., Coto-Montes, A., Boga, J.A., Andersen, L.P., Zhou, Z., Galano, A., Vriend, J., Tan, D.X., & Reiter, R.J.. (2015). Melatonin: an ancient molecule that makes oxygen metabolically tolerable. J. Pineal Res., 59 (4), 403-419. DOI: https://doi.org/10.1111/jpi.12267
Khara, M.R., & Cuchyrka, L.I. (2014). Perekysne okysnennia lipidiv u miokardi pry rozvytku nekrotychnoho protsesu na tli melatoninu ta riznoi aktyvnosti honad [Lipid peroxidation in the myocardium during the development of a necrotic process on the background of melatonin and different activity of the gonads]. Klin. ta eksperem. Patol. – Clinical and Experimental Pathology, 4 (50), 148-151 [in Ukrainian].
Khara, M.R., & Pelykh, V.Ye. (2010). Osoblyvosti perekysnoho okysnennia lipidiv ta antyoxydantnoi systemy u honadektomovanykh samok shchuriv pry modeliuvanni nekrotychnoho poshkodzhennia miokarda ta korektsii [Peculiarities of lipid peroxidation and antioxidant system in gonadectomized female rats in modeling of necrotic myocardial injury and correction]. Med. khimiia – Medical Chemistry, 1, 80-84. [in Ukrainian].
