INFLUENCE OF L-ARGININE AND AMINOGUANIDIN ON THE LEVEL OF SYNTHESIS OF NITRIC OXIDE IN THE CEREBELLUM AND CEREBRAL HEMISPHERES IN EXPERIMENTAL ANTIPHOSPHOLIPID SYNDROME
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i4.10696Keywords:
antiphospholipid syndrome, pregnancy, nitric oxide, cerebellum, cerebral hemispheresAbstract
Introduction. Antiphospholipid syndrome (APS) is an autoimmune disease characterized by the presence of antiphospholipid antibodies, arterial and venous thrombosis, thrombocytopenia, miscarriage.
The aim of the study – to investigate the effect of the combined effect of L-arginine and aminoguanidine on the content of nitrite anions (NO2–) and nitrate anions (NO3–) in the cerebellum and cerebral hemispheres of BALB/c mice with APS before pregnancy and on the 18th day of pregnancy.
Research Methods. The study was performed on female BALB/c mice, for which APS was simulated. L-arginine (25 mg/kg) in combination with aminoguanidine (10 mg/kg) was used for correction. The content of NO in the homogenates of cerebellum and cerebral hemispheres of the animals with APS before pregnancy and on the 18th day of pregnancy was determined by the number of its stable metabolites of nitrite anions (NO2–) and nitrate anions (NO3–).
Results and Discussion. The content of stable metabolites of nitric oxide NO2– and NO3– in the cerebellum and cerebral hemispheres increased in the BALB/c mice with APS compare to the intact animals. In cases of experimental APS, on the 18th day of pregnancy, an increase in the content of NO2– and NO3– in the cerebellum was evidenced, as well as a decrease in NO2– and NO3– in the brain hemispheres of the animals with APS compare to the controls. In case of the combined administration of precursor of synthesis of NO L-arginine and a selective inhibitor of iNOS aminoguanidine into the animals with APS, a decrease in the content of NO2– and NO3– in the cerebellum (by 28 % and 17 %) and cerebral hemispheres (by 16 % and 10 %) was evidenced comparing to the animals with APS. In the pregnant BALB/c mice with APS administered with L-arginine in combination with aminoguanidine, a decrease of NO2– by 29 % and NO3– by 25 % in cerebellum, an increase of NO2– by 21 % and NO3–by 22 % in the cerebral hemispheres was evidenced comparing to those of the pregnant females with APS.
Conclusions. It has been established that the combined use of the precursor of synthesis of NO L-arginine and a selective inhibitor iNOS aminoguanidine leads to normalization of the level of stable metabolites of NO2– and NO3– nitric oxide synthesis in the cerebellum and cerebral hemispheres of the BALB/c mice with APS before pregnancy and on the 18th day of pregnancy.
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