THE INFLUENCE OF SKUTELLARIA BAIKALENSIS ON THE LEVEL OF MACRO- AND MICROELEMENTS AND THE INDEXES OF LIPID METABOLISM IN RATS TO ADRENALINE STRESS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2019.v.i3.10562Keywords:
adrenaline stress, Scutellaria baikalensis, macro- and trace elements, blood, liverAbstract
Introduction. Stress and its consequences are a serious health problem for a modern human. There are conducted intensive research for ways to reduce the burden on the body. Despite the use the drug Skutellaria in the clinical practice, the metabolic effects of this drug remain insufficiently investigated.
The aim of the study – to evaluate the effect of the extract from the Scutellaria baikalensis on the level of essential elements – Calcium, Magnesium, Kuprum and Zinc in rats with an adrenaline stress pattern at different intervals.
Research Methods. For the modeling of adrenalin stress we used two-fold intramuscular administration of 0.18 % adrenaline hydrochloride solution with dose of 0.05 mg/kg body weight. The daily dose of Scutellaria baikalensis for the rat was 0.3 ml/kg, introduced orally for 30 minutes prior to the injection of adrenaline. Five groups of animals were investigated: I – control, which were injected with 0.9 % of NaCl solution; II and IV – animals injected with adrenaline twice with 1 h interval; III and V groups – animals injected twice with adrenaline at intervals of 1 hour, and twice with Scutellaria baikalensis extract for 30 minutes before the injection of adrenaline. Collection of material was conducted through 30 minutes and 24 hours. The content of adrenaline was determined by colorimetric method; the content of kuprum, zinc, magnesium, calcium – by atomic adsorption spectrophotometry.
Results and Discussion. The obtained results testify about the development of dysmicroelementosis in the organism of experimental animals in the conditions of adrenalin stress, which is accompanied by the redistribution of macro elements – Calcium and Magnesium and trace elements – Kuprum,and Zinc in the blood and liver. Such changes in the level of essential elements have a significant effect on the regulation of metabolic processes and the level of adrenaline. The use of the Scutellaria baikalensis drug before the introduction of adrenaline leads to an increase in the level of Calcium, Kuprum,in the blood and liver compared with the control group and the animals that did not receive the drug. The Magnesium level was characterized by different directions of variation, depending on the observation period. Zinc content was within the control group values and declined relative to adrenaline groups.
Conclusion. The results of the research indicate that the extract from the Scutellaria baikalensis in conditions of adrenaline stress contributes to maintaining the homeostasis of essential elements – Calcium, Magnesium, Kuprum and Zinc.
References
Wasowski, C. & Marder, M. (2012). Flavonoids as GABAA receptor ligands: the whole story? J. Exp. Pharmacol., 4, 9-24.
van der Kooij, M.A., Jene, T., Treccani, G., Miederer, I., Hasch, A., Voelxen, N., & Müller, M.B. (2018). Chronic social stress-induced hyperglycemia in mice couples individual stress susceptibility to impaired spatial memory. Proc. Natl. Acad. Sci. U. S. A., 115 (43), E10187-E10196. DOI: https://doi.org/10.1073/pnas.1804412115
Liu, L., Dong, Y., Shan, X., Li, L., Xia, B. & Wang, H. (2019). Anti-depressive effectiveness of baicalin in vitro and in vivo. Molecules, 24 (2), E326. doi: 10.3390/molecules24020326. DOI: https://doi.org/10.3390/molecules24020326
Zapadnyuk, I.P., Zapadnyuk, V.I., Zakhariia, E.A., & Zapadnyuk, B.V. (1983). Laboratornye zhivotnye. Razvedenie, soderzhanie, ispolzovanie v eksperimente [Laboratory animals. Breeding, keeping, and use in an experiment]. 3rd ed. Kyiv: Vyshcha shkola [in Russian].
Chen, M.H., Lu, J.Y., Xie, L., Zheng, J.H. & Song, F.Q. (2010). What is the optimal dose of epinephrine during cardiopulmonary resuscitation in a rat model? Am. J. Emerg. Med., 28 (3), 284-290.
Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. (2010). Official Journal of the European Union, L273, 33-79.
Fukuda, A., Muramatsu, K., Okabe, A., Shimano, Y., Hida, H., Fujimoto, I. & Nishino, H. (1998). Changes in intracellular Ca2+ induced by GABAA receptor activation and reduction in Cl– gradient in neonatal rat neocortex. J. Neurophysiol., 79 (1), 439-446. DOI: https://doi.org/10.1152/jn.1998.79.1.439
Pan, Z.H & Lipton, S.A. (1995). Multiple GABA receptor subtypes mediate inhibition of calcium influx at rat retinal bipolar cell terminals. J. Neurosci., 15 (4), 2668-2679. DOI: https://doi.org/10.1523/JNEUROSCI.15-04-02668.1995
Obrietan, K. & van den Pol, A.N. (1998). GABAB receptor-mediated inhibition of GABAA receptor calcium elevations in developing hypothalamic neurons. J. Neurophysiol., 79 (3), 1360-1370. DOI: https://doi.org/10.1152/jn.1998.79.3.1360
Iulish, E.I. (2007). Rol magniya v norme i patologii [The role of magnesium in the norm and pathology]. Zdorovye rebenka – Child Health, 5 (8), 49-52 [in Russian].
Tsygan, V.N., Skalnyy, A.V. & Mokeyeva, E.G. (2012). Sport, immunitet, pitanie [Sport, immunity, nutrition]. Saint-Petersburg: ELBI-SPb [in Russian].
Yeshchenko, Yu.V. (2009). Zminy vmistu tsynku v klitynakh pry stresi [Cell zinc content during stress]. Fiziologichnyi zhurnal – Physiological Journal, 55 (5), 72-78 [in Ukrainian].
