COMPARATIVE ANALYSIS OF VOLATILE COMPOUNDS OF LEAVES OF COLEWORT HEART-LEAVED AND COLEWORT KOKTEBELICA
Keywords:
colewort heart-leaved, colewort Koktebelica, leaves, volatile compounds, gas chromatographic mass spectrometryAbstract
Introduction. Inappropriate use of natural resources leads to an annual decrease in reserves of wild plants and encourages the use of cultivated flora plants. These plants include the colewort heart-leaved (Crambe cordifolia Steven) and the colewort Koktebelica (Crambe koktebelica (Junge) N. Busch), which, according to literature sources, have antimicrobial properties and are the source of natural antioxidants. Volatile compounds are one of the classes of biologically active compounds that manifest antimicrobial and antioxidant activity.
The analysis of scientific literature sources showed the lack of information about volatile compounds of the colewort heart-leaved and the colewort Koktebelica, therefore the aim of our study was to learn and compare the volatile compounds of the leaves of these plant species.
Research Methods. Determination of the component composition of volatile compounds was carried out by the method of gas chromatographic mass spectrometry on the chromatograph Agilent Technology 6890N. To identify the components of the works, the library of mass spectra of NIST 02 was used.
Results and Discussion. As a result of the conducted studies, 33 components of volatile compounds were detected in the colewort heart-leaved, of which 25 were identified; in the colewort Koktebelica there are 28 components, 15 identified. A comparative analysis of the volatile compounds of the investigated plant species showed that they have 11 identical components, which can be marker compounds of volatile fractions of plants of the genus Colewort (Crambe L.).
Conclusions. For the first time, the method of gas chromatographic mass spectrometry was used to study the composition of the volatile compounds of the colewort heart-leaved (Crambe cordifolia Steven) and the colewort Koktebelica (Crambe koktebelica (Junge) N. Busch), leaves, in which 33 components of the volatile compounds, of which 25 were identified, and among 28 components – 15 identified, respectively. There are eleven components common to both species: β-farnesene, β-ionone, bisabolol oxide A, dibutyl phthalate, phyton, 3-methyl-2- (3,7,11- trimethyl dodecyl) furan, n-hexadecanoic acid, phytol, 2- ethylhexylhydrogenophthalate, heneicosane, nonacosane.
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