PECULIARITIES OF OXIDATIVE PROCESSES AT EXPERIMENTAL NONPENETRATING MECHANICAL CORNEAL TRAUMA INJURY
Introduction. Disorganization of peroxidation processes plays an important role in the pathogenesis of a mechanical nonpenetrating corneal injury , but these interrelationships were not been studied sufficiently, which led us to study the proper direction of this.
The aim of the study – to investigate the changes in the intensity of peroxide oxidation of lipids, proteins, and the content of nitrogen monooxide metabolites in rabbit blood under conditions of mechanical nonpenetrating corneal injury in dynamics.
Materials and Methods. Experimental studies were carried out on mature rabbits of the Chinchilla breed. For the study of the features of free radical oxidation at experimental mechanical nonpenetrating eye injury spectrophotometrically, the concentration of hydroperoxides, the content of TBA-reactants, degree of oxidative modification of proteins, level of metabolites of nitrogen monoxidewere determined.
Results and Discussion.It was established the increase in the content of proteins and lipid peroxidation products in rabbits during the mechanical nonpenetrating corneal injury. The results of our study indicate a wave-like dynamics of changes in end products of nitrogen monoxide in serum of rabbits with mechanical non-penetrating corneal injury.
Conclusions. Under the condition of an experimental non-penetrating corneal injury, there is a significant increase of lipid peroxidation (increased levels of hydroperoxides and TBA-active products) and oxidation modification of proteins (aldehyde- and ketone derivatives of dinitrophenylhydrazones of neutral and basic nature), compare to control, with minimum values by 7 days and maximum – by 14 days (p<0.05).Experimental non-penetrating corneal injury is accompanied by a decrease in the level of end products of nitrogen monoxide by 3–7 days on average by 26.0%, followed by an increase of 10 and 14 days, respectively, by 8.0% and 24.9%, relative to the control group ( p<0.01).
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