STUDY OF AN ENZYME ANTIOXIDANT PROTECTION IN RATS IN CASE OF TOBACCO SMOKE COMBINED WITH PROLONGED ADMINISTRATION OF MONOSODIUM GLUTAMATE IN THE SEX AND AGE ASPECTS
Introduction. The widespread prevalence of tobacco smoking is a global problem of humanity, the solution of which is directed at the efforts of many scientists and professionals. At the same time, the distinctive feature of modern food technologies is the use of nutritional supplements. One of the most common nutritional supplements in Ukraine and in Europe is glutamate sodium (E621), which is not always safe for human health.
The aim of the study – to investigate the state of the enzyme level of the antioxidant system in rats during “passive tobacco smoking” on the basis of prolonged administration of monosodium glutamate in the sex and age aspects.
Research Methods. The study was conducted on 96 white, non-linear, sexually mature and sexually immature rats of both sexes. Each group of animals was divided into four subgroups: I – control; ІІ – rats, which were modeled “passive tobacco smoking”; ІІІ – rats, which were given monosodium glutamate; IV – rats, which were modeled “passive tobacco smoking” against the background of the introduction of monosodium glutamate.
Results and Discussion. Excessive formation of products of free radical oxidation under the condition of isolated effect of tobacco smoke causes the extinction of the antioxidant potential of blood and lung tissues, which is manifested by a significant decrease in superoxide dismutase activity in erythrocytes hemolysates by 28.8 % vs the control group, and in the supernatant of the lung homogenate – by 53.1 % (p<0.001) respectively. “Passive tobacco smoking” against the background of monosodium glutamate application is accompanied by a significant decrease in superoxide dismutase activity (by 41.0 % (p<0.001)) relative to the control group, which is by 17.1 % (p<0.02) below this index in case of the isolated effect of tobacco smoke in erythrocytes hemolysates and 58.7 % in the supernatant of lung homogenate, which is 12.0 % (p<0.02) below this index, provided that the tobacco smoke is isolated. Unidirectional changes were observed with catalase activity.
Conclusions. In case of exposure to tobacco smoke, extinction of the antioxidant potential (reduction of superoxide dismutase and catalase activity) in the blood and lung tissue is observed, which is more pronounced in the case of “passive tobacco smoking” combined with monosodium glutamate application. In the sexual aspect, the indices of antioxidant protection in the context of “passive tobacco smoking” against the use of monosodium glutamate are more pronounced in females, and with the age-old comparison of changes in the activity of these enzymes established their more intense decline in immature rats.
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