BIOCHEMICAL MARKERS OF MORPHOLOGICAL CHANGES IN MYOCARDIUM OF RATS IN ITS ADRENALIN-CALCIUM DAMAGE
Introduction. Myocardial infarction is a dynamic process accompanied by the transition of reversible changes to irreversible ischemic injury and completes the replacement of the reduced part of the myocardium by a fibrous scar.
The aim of the study– to determine the relationship between changes in lipid peroxidation products, oxidative modification of proteins (OMP), nitrite anion and morphological disorders in the heart of animals with adrenalin-calcium model (ACM) of its lesion and correction of the pathological process by quercetin (Quе).
Research Methods.Experiments were performed on 195 Wistar male rats, 5–6 months old. Rats were injected with adrenaline hydrotartrate and calcium gluconate, for correction – quercetin. In the heart, diene conjugates (DC), TBA-active products, OMP, nitrite anion were determined. Histologic preparations were studied by staining with hematoxylin and eosin, by Heidgenheim, and they were also seen in polarized light.
Results and Discussion.At ACM, cardiac involvement in the growth of DC and TBA-active products was greatest after 7 days and 14 days, OMP– after 24 hours, 3 days (maximum), 7 days. The concentration of nitrite anion was high from 24 hours to 21 days (after 3 days and 14 days, the peaks were noted). Quercetin contributed to reducing the content of OMP after 7, 14 and 28 days, DC – 7, 14 and 21 days, TBA-active products, nitrite anion – in all terms of the study. In the morphological study revealed a violation of tincter properties, the appearance of necrosis, contracture. Changes grew with an increase in the duration of the research and corresponded to deviations of biochemical indicators. Quercetin prevents the onset of lesions.
Conclusions.The revealed morphological changes confirmed the data of biochemical parameters. Morphological changes grew with an increase in the duration of the study and corresponded to the deviation of biochemical parameters. Quercetin reduced the lesion.
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