Optimization of human somatic cells cryopreservation protocols by polyethylene glycols

  • O. M. Perepelytsina
  • A. P. Ugnivenko
  • D. P. Burlaka
  • S. V. Bezugly
  • M. V. Sydorenko
Keywords: cryopreservation, vitrification, polyethylene glycol, cooling rate, cell survival, freezing protocols.

Abstract

Using of polyethylene glycol in optimization of human somatic cells of cryopreservation protocols was analyzed. Low molecular weight PEG as vitrification solution supplement exhibited a high cooling speed and provided cell survival in 200 % comparing with control. It allows recommending the use of low molecular weight PEG in vitrification environment for effective cell vitrification protocols.

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Published
2016-11-21
How to Cite
Perepelytsina, O. M., Ugnivenko, A. P., Burlaka, D. P., Bezugly, S. V., & Sydorenko, M. V. (2016). Optimization of human somatic cells cryopreservation protocols by polyethylene glycols. Medical and Clinical Chemistry, (3), 5-12. https://doi.org/10.11603/mcch.2410-681X.2016.v0.i3.6933
Section
ORIGINAL INVESTIGATIONS