CAROTENOIDS CONTENT IN THE INFLORESCENCES OF TAGETES PATULA L.
DOI:
https://doi.org/10.11603/mcch.2410-681X.2024.i1.14446Keywords:
French Marigold, variety “Super Hero Orange Flame”, carotenoidsAbstract
Introduction. Plants of the Asteraceae family of the genus Tagetes L. (Marigold) are considered highly potent raw materials for the development of new remedies. The therapeutic activity of Tagetes L. is based on some bioactive compounds, such as carotenoids, present in their tissues. Among others, the most promising carotenoid-containing species are T. patula nana L. var. Gold Kopfen and T. patula L. var. Superboy Orange. Despite massive data about the content of carotenoids in different varieties of T. patula L., the content of carotenoids in some newly developed varieties, including “Super Hero Orange Flame” (by Benary, Germany), still needs to be determined.
The aim of the study – to determine a quantitative content of carotenoids in the inflorescences of Tagetes рatula L., variety “Super Hero Orange Flame”.
Research Methods. The study was conducted on the air-dry inflorescences of the cultivated T. рatula L. plants (variety “Super Hero Orange Flame”) collected during blooming at the experimental plot of Bukovynian State Medical University (Chernivtsi, Ukraine). Thin-layer chromatography (TLC) and spectrophotometry of the extractions obtained using hexane and 96 % ethanol were employed to find a qualitative composition of carotenoids, and UV-spectroscopy was used to establish their quantitative content. The total content of carotenoids in the mixture was recalculated to β-carotene using an internal standard.
Results and Discussion. A mixture of carotenoids was analyzed in the hexane and ethanolic extractions from the inflorescences. As a result, at least seven and ten compounds were identified in the hexane and ethanolic extractions, respectively. All these compounds are identified in the lipophilic eluents and exhibit reductive properties. The spectrophotometrical analysis of an extraction obtained after treating the mixture with the 96 % ethanol followed by a 1:5 dilution of the extraction with the same ethanol showed two distinct absorption bands with maximums at 442 and 468 nm, which proves the presence of some carotenoids in the extraction. A visible-band absorption pattern of the hexane extraction is also typical for a mixture of carotenoids.
The UV-spectrometry proves that the mass content of carotenoids in the mixture is (440±0.32) mg% of dry weight as recalculated to β-carotene.
Conclusions. It has been found that the inflorescences of the species Tagetes patula L., genus Tagetes, variety “Super Hero Orange Flame”, collected at the experimental plot of Bukovynian State Medical University, contain a significant amount of carotenoids (440±0.32) mg% of dry weight. Therefore, this variety can be further studied as a potential source of carotenoids.
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