DYNAMICS OF THE ENZYME LINK INDICATORS OF ANTIOXIDANT PROTECTION IN THE LUNGS UNDER THE INFLUENCE OF EXPERIMENTAL ACUTE BLOOD LOSS DEPENDING ON THE TYPE OF BLOOD REPLACEMENT
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i3.14162Keywords:
acute blood loss, lungs, catalase, superoxide dismutase (SOD), correctionAbstract
Introduction. Acute blood loss is one of the main causes of an injured body’s death. Hypoperfusion of organs and the development of hypoxia cause disturbances in the antioxidant protection system. Balanced crystalloids are used for correction. Recently, antioxidants have been used in the complex.
The aim of the study – to find out the dynamics of the enzyme link of antioxidant protection in the lungs under the influence of acute blood loss and to evaluate the effectiveness of blood replacement with a balanced crystalloid in combination with an antioxidant.
Research Methods. Experiments were performed on 114 white male Wistar line rats. Animals were divided into four groups: group 1 – control (intact); group 2 – acute blood loss in the amount of 2 % of body weight; group 3 – acute blood loss and correction with Ringer’s lactate solution in a ratio of 1:1; group 4 – acute blood loss and correction with Ringer’s lactate solution in a 1:1 ratio in combination with 2-ethyl-6-methyl-3-hydroxypyridine succinate at a dose of 100 mg·kg-1. Means for correction were injected once at 60 minutes after simulation of acute blood loss. After 1, 3 and 7 days, superoxide dismutase and catalase activity was determined in the lung parenchyma.
Results and Discussion. It was established that acute blood loss in the amount of 2 % of body weight compared to the control group is accompanied by a compensatory increase in superoxide dismutase activity of lung after 3 and 7 days of the experiment and catalase activity after 3 days of the experiment. The injection only with Ringer’s lactate solution compared to rats without correction after 3–7 days of the experiment caused a significant decrease in SOD activity of lungs, and after 3 days – catalase activity. The use of Ringer’s lactate solution in combination with 2-ethyl-6-methyl-3-hydroxypyridine succinate, on the contrary, is accompanied by an increase in SOD activity of the lungs after 3 and 7 days of the post-traumatic period and catalase activity – after 1 and 7 days compared to rats with monotherapy with the Ringer’s lactate solution, which contributes to the growth of the body’s antioxidant reserve and may have a certain practical value in providing a sanogenic effect on the body and increase in rat’s survival after acute blood loss.
Conclusions. Against the background of acute blood loss in the amount of 2 % of body weight, activation of the enzyme link of antioxidant protection takes place in the lungs with a maximum after 7 days of the post-traumatic period, catalase activity – after 3 days. A single intravenous injection with Ringer’s lactate solution is accompanied by a decrease in SOD activity in lungs after 7 days, catalase activity – after 3 days of the experiment. A single intravenous injection with Ringer’s lactate solution in combination with 2-ethyl-6-methyl-3-hydroxypyridine succinate compared to crystalloid monotherapy causes an increase in SOD activity after 3-7 days of the experiment, catalase activity – after 1 and 7 days.
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