DETECTION OF MIANSERIN IN URINE BY CHROMATOGRAPHIC METHODS

Abstract

 Introduction. According to the literature, the greatest toxicological danger is formed by drugs that affect the central nervous system. According to toxicology services, about 800,000 fatal poisonings by psychotropic, hypnotic, sedative, antidepressant and other groups of drugs are registered annually in the world. In recent years, scientific interest in the study of antidepressants and narcotic substances in toxicological practice has grown significantly. However, every medicinal substance under certain conditions can become a poison, especially those that have a nature of long-term use, such as the tricyclic antidepressant mianserin.

The aim of the study – to develop methods for the identification of mianserine in biological fluids (urine) in the joint presence of thin layer chromatography (TLC), high performance liquid chromatography (HPLC), gas-liquid chromatography with mass spectrometry (Gl-MS).

 Research Methods.The research used: chloroform solutions of mianserin and its metabolite desmethylmianserin M-7 isolated from the urine of poisoned animals; chromatographic systems: toluene-acetone-96 % ethyl alcohol-25  % ammonia solution (45: 45: 7,5: 2,5), chloroform-acetone (10:10), ethyl acetate-methanol-25 % ammonia solution (85: 10: 5), ethanol-methylene chloride- 25% ammonia solution (57.5: 40: 2.5); Marki and Dragendorf reagents; gas chromatograph Agilent 1200.

Results and Discussion. Identification of mianserine was performed by identification reactions, thin layer chromatography (TLC). The optimal chromatographic system is ethanol 96 %: methylene chloride: 25 % ammonia solution (57.5: 40: 2.5); developer – Mandeline reagent. The parameters of substance identification in these conditions are established: retention time, spectral ratios of substances, absorption spectra in UV spectra.

Conclusion. A method for detecting mianserine by TLC on Sorbfil PTSH plates has been developed. A method for the determination of mianserine using HPLC has been developed. A method for the identification of mianserine by gas-liquid chromatography with mass spectrometry (Gl-MS) has been developed. The optimal conditions for the isolation of mianserine in the joint presence of model mixtures of bioliquid (urine).