THE ROLE OF LIPID PEROXIDATION PROCESSES IN THE DEVELOPMENT OF OVARIAN PATHOLOGY IN RATS AND THEIR CORRECTION WITH INOSITOL DRUGS

Abstract

Introduction. Among the urgent problems of today is the problem of infertility. Risk factors for its development include: age after 40 years; hypodynamia, stress, toxic effects of lead. At the same time, cell membranes, which include phospholipids and proteins, are destroyed. The use of inositol contributes to their restoration, which, accordingly, improves reproductive function.

The aim of the study – to evaluate the development of oxidative stress in female rats with ovary pathology and the correction it with inositol (In).

Materials and Methods. Experiments were performed on 216 white female Wistar rats, which were divided into 4 series: 1 – control, 3.5–4 months old rats, 2 – rats 7.5–8 months old, 3 – rats that were intragastrically injected with acetate lead solution for 15 days in a dose of 0.05 mg/kg, 4 – hypodynamic stress. There were 6 groups of animals in each series: A – control (C), B – In, C – FT 500 plus (FT), D – pregnancy, E – In + pregnancy, F – FT + pregnancy. In and FT were introduced intragastrically for 15 days at the rate of 400 mg/kg of In. In the ovaries of animals, the concentration of active and triene conjugates (DC, TC), TBA-active products (TBA-ap), Schiff's base (SB), activity of superoxide dismutase (SOD) and catalase (Cat) was determined.

Results and Discussion. In 1 series of rats, it caused an increase in the ovaries of DC, TC. In the 2nd series, compared to K, increased DC, TC, SB, decreased SOD, th was in In action too. In decreased Cat, FT – increased DC, SB, decreased TBA-ap, SOD, Cat. In the 3rd series, compared to K, DC, TC, SB, TBA-ap, Cat increased, SOD decresed. In reduced DC, TC, SB, TBA-ap, increased SOD, FT reduced DC, TC, SB, TBA-ap, Cat, increased SOD. In the 4th series, compared to K, DC, TC, SB, TBA-ap, Cat increased, SOD decresed. In increased DC, TC, SB, SOD, Cat, FT – decreased DC, TC, increased SOD. In the K series of parturient females, which were introduced to any of the In, TC, FT – DC increased, TC decreased. In and FT reduced SB and TBA-ap. In decreased SOD and Cat, FT – increased Cat. In series 2, DC, TC, SB, TBA-ap, SOD were decreased, Cat – increased. In increased DC, TC, SB, TBA-ap. SOD, Cat, FT – DC, SB, TBA-ap, SOD, Cat. In series 3, DC, SB, Cat increased, TBA-ap decreased. In decreased SB, Cat, increased SOD, FT – increased DC, TC, TBA-ap, FT  – decreased SB. In series 4, DC, TC, TBA-ap, Cat accumulated, SOD decreased. In increased DC, SB, Cat, decreased TC, TBA-ap. FT reduced DC, TC, TBA-ap, increased SB, SOD, Cat.

Conclusion. Inositol at a dose of 400 mg/kg is inappropriate to use in an experiment in healthy young female rats. In 7.5–8 month old males, in animals exposed to a nanodose of lead acetate, hypodynamic stress is optimal for reducing lipid breakdown, both inositol and FT 500 plus, although the latter is more effective. Effectiveness from pre-pregnancy use of both inositol and high FT 500 plus.