EFFECT OF GADOLINIUM ORTHOVANADATE NANOPARTICLES WITH EUROPIUM GDVO4:EU3+ WITH AND WITHOUT PREVIOUS UV IRRADIATION ON THE GENERATION OF REACTIVE OXYGEN SPECIES IN RAT LEUKOCYTES
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i3.14107Keywords:
gadolinium orthovanadate, nanoparticles, oncology, reactive oxygen species, flow cytometryAbstract
Introduction. Considering the significant rate of oncopathologies spreading, search and research of methods for increasing the effectiveness and safety profile of anticancer therapy is relevant. One of the promising radiosensitizers are nanoparticles, including gadolinium orthovanadate with europium. However, data on their cytotoxicity are quite limited, which determines the feasibility of their further study.
The aim of the study – to evaluate the generation of reactive oxygen species (ROS) in rats peripheral blood leukocytes under the influence of GdVO4:Eu3+ nanoparticles (oral administration).
Research methods. The WAG rats were used in our study, which were divided into 3 groups that received drinking water (group 1); aqueous solution of GdVO4:Eu3+ at a dose of 50 μg/kg of body weight without UV irradiation (group 2) and with previous UV irradiation (group 3) for 14 days intragastrically. In the suspension of leukocytes, the generation of ROS was determined using the fluorescent probe 2,7-dichlorodihydrofluorescein diacetate on a flow cytometer. The obtained results were processed statistically.
Results and Discussion. The results indicate uneven generation of ROS in leukocytes: a significant intensification of ROS production was observed in group 2, compared to the control. Previous UV irradiation of GdVO4:Eu3+ nanoparticles led to a decrease in the fluorescence index (indication of ROS generation) (group 3), compared to the other two groups. It is obvious that the determining factor is not only the UV irradiation of nanoparticles, but also the duration and method of administration of the nanoparticles themselves.
Conclusions. The results of our research indicate that GdYVO4:Eu3+ nanoparticles administered orally in rats at a dose of 50 μg/kg of body weight without previous UV irradiation are able to reliably increase the generation of ROS in leukocytes, while the use of nanoparticles with a similar dose with previous UV irradiation, on the contrary, is accompanied by a decrease in ROS production, even compared to the control.
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