THE CONTENT OF INTERLEUKIN-1β, PROCALCITONIN IN THE SERUM OF PATIENTS WITH LACTATIONAL MASTITIS

Authors

  • N. R. Demianchuk DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • V. M. Akimova DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • М. М. Shchurko DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • N. Z. Lutsiv DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • L. D. Soyka DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • B. M. Beliavska DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • L. Y. Lapovets DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • O. Y. Buchko DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY
  • S. O. Tkachuk DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2023.i2.13972

Keywords:

interleukin-1β, procalcitonin, lactostasis, lactational mastitis

Abstract

Introduction.  Favorable factors for the development of the inflammatory process in the mammary gland are lactostasis, the presence of pathogenic flora, as well as a decrease in the immunobiological reactivity of the woman’s body. The inflammatory process is driven and regulated by a number of mediators. In particular, pro-inflammatory interleukin-1β induces an inflammatory reaction and an acute phase response, and carries out the interaction between nonspecific and specific links of immunity. Procalcitonin is considered the most promising indicator of the septic process, which allows for a differential diagnosis of bacterial and non-bacterial inflammation, for assessing the severity of the patient's condition and the effectiveness of anti-inflammatory therapy.

The aim of the study – to assess the content of interleukin-1β, procalcitonin in the serum of patients with lactational mastitis.

Research Methods. The blood serum of 97 women aged 18 to 36 have been examined (average age: 26±5 years old). The control group consisted of 30 practically healthy lactating women, the first group included 30 women with lactostasis, the second group – 37 women who developed lactational mastitis. The contents of interleukin-1β and procalcitonin were determined by enzyme immunoassay using an automatic STAT FAX 303 plus analyzer.

Results and Discussion. The content of IL-1β in the serum of women of group I ((6.37±0.46) pg/ml) is 3.5 times higher than that of the control group ((1.78±0.11) pg/ml, p<0.05). The average concentration of IL-1β in group II is (8.32±0.38) pg/ml, which is 4.7 times higher than the control values and exceeds the indicator of women in group I by 1.3 times (p<0.05). With the development of lactostasis, the PCT content ((0.07±0.02) ng/ml) is 3.3 times higher than the control value ((0.021±0.004) ng/ml, p<0.05). In the serum of women of group II, the PCT content is (0.81±0.25) ng/ml, which exceeds the value of this marker in the control group and group I by 38.6 and 11.6 times, respectively (p<0.05).

Conclusions. The established concentration of IL-1β confirms the role of this cytokine in the protective reaction and can be used to assess the activity of the inflammatory process in lactostasis and lactational mastitis. Determining procalcitonin as a marker of bacterial infection is appropriate for the prognosis of the septic process in the mammary gland.

References

Lai, B.-Yo., Yu, B.-W., Chu, A.-J., Liang, S.-B., Jia, L.-Y., Liu, J.-P., ... Pei, X.-H. (2021). Risk factors for lactation mastitis in China: A systematic review and meta-analysis. PLoS One, 16 (5). DOI: 10.1371/journal.pone.0251182.

Salmanov, A.G., Savchenko, S.E., Chaika, K.D., Vitiuk, A., Ruban, I., Dyndar, A.O., … Abbasova, E. (2020). Postpartum mastitis in the breastfeeding women and antimicrobial resistance of responsible pathogens in ukraine: results a multicenter study. Wiad Lek., 73 (5), 895-903. PMID: 32386365.

Młodawska, М., Młodawski, J., Pazera, G., Roki­ta, W. (2019). Breast is the best ‒ czylico każdy ginekolog o karmieniu piersią wiedzieć powinien. Ginekologia i Perinatologia Praktyczna, 4 (1), 23-33.

Rimoldi, S.G., Pileri, P., Mazzocco, M.I., Rome­ri, F., Bestetti, G., Calvagna, N., … Cetin, I. (2020). The role of Staphylococcus aureus in mastitis: A Multidisciplinary Working Group Experience. J. Hum. Lact., 36 (3), 503-509. DOI: 10.1177/0890334419876272.

Wilson, E., Woodd, S.L., Benova, L. (2020). Incidence of and risk factors for lactational mastitis: A systematic review. J. Hum. Lact., 36 (4), 673-686. DOI: 10.1177/0890334420907898.

Mantovani, A., Dinarello, C.A, Molgora, M., Gar­landa, C. (2019). Interleukin-1 and Related Cytokines in the Regulation of Inflammation and Immunity. Immunity, 50(4), 778-795. DOI: 10.1016/j.immuni.2019.03.012.

Dinarello, C.A. (2000). Proinflammatory cytokines. Ches., 118 (2), 503-508. DOI: 10.1378/chest.118.2.503.

Samsudin, I., Vasikaran, S. (2017). Clinical utility and measurement of procalcitonin. Clin. Biochem. Rev., 38 (2), 59-68. PMID: 29332972.

Cao, X. E., Ongagna-Yhombi, S. Y., Wang, R., Ren, Y., Srinivasan, B., A Hayden, J., ... Mehta, S. (2022). A diagnostic platform for rapid, simultaneous quantification of procalcitonin and C-reactive protein in human serum. E.BioMedicine, 76, 103867. DOI: 10.1016/j.ebiom. 2022.103867.

Xu, H.-G., Tian, M., Pan, Sh.-Y. (2022). Clinical utility of procalcitonin and its association with pathogenic microorganisms. Crit. Rev. Clin. Lab. Sci., 59 (2), 93-111. DOI: 10.1080/10408363.2021.1988047.

Boraschi, D. (2022). What is IL-1 for? The functions of Interleukin-1 across evolution. Front. Immu­nol., 13, 872155. DOI: 10.3389/fimmu.2022.872155.

Giai, C., Gonzalez, C., Sabbione, F., Garofalo, A., Ojeda, D., O Sordelli, D., ... I Gómez, M. (2016). Sta­phylococcus aureus Induces Shedding of IL-1RII in Monocytes and Neutrophils. J. Innate Immun., 8 (3), 284-298. DOI: 10.1159/000443663.

Van Den Eeckhout, B., Tavernier, J., Gerlo, S. (2021). Interleukin-1 as innate mediator of T cell immunity. Front. Immunol., 11, 621931. DOI: 10.3389/fimmu. 2020.621931.

Supino, D. Minute, L., Mariancini, A., Riva, F., Magrini, E., Garlanda, C. (2022). Negative Regulation of the IL-1 System by IL-1R2 and IL-1R8: Relevance in pathophysiology and disease. Front. Immunol., 13, 804641. DOI: 10.3389/fimmu.2022.804641.

Demianchuk N.R., Beliavska B.M., Lapo­vets L.Ye., Kunina I.O. (2015). Concentration of IL-1β in blood serum and breast milk with lactation mastitis. Journal of Problems of Biology and Medicine, 2, 1 (118), 118-120 [in Ukrainian].

Póvoa, P., Coelho, L., Dal-Pizzol, F., Ferrer R., Huttner, A., Conway Morris, A., … Kalil, A. (2023) How to use biomarkers of infection or sepsis at the bedside: guide to clinicians. Intensive Care Med., 49 (2), 142-153. DOI: 10.1007/s00134-022-06956-y.

Zhang, Y., Zhang, J., Zhao, L., Zhao, N., Zhang, Y., Zhang, J., Wang S. (2021). Procalcitonin in infectious diseases: a bibliometric analysis. Ann. Palliat. Med., 10 (7), 8003-8014. DOI: 10.21037/apm-21-1607.

Varela-Patino, M., Lopez-Izquierdo, R., Velayos-Garcia, P., Alvarez-Manzanares, J., Ramos-Sanchez, C., Carbajosa-Rodriguez, V., ... Eiros, J.M. (2020). Usefulness of infection biomarkers for diagnosing bacteremia in patients with a sepsis code in the emergency department. Infez. Med., 28 (1), 29-36. PMID: 32172258.

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Published

2023-07-11

How to Cite

Demianchuk, N. R., Akimova, V. M., Shchurko М. М., Lutsiv, N. Z., Soyka, L. D., Beliavska, B. M., … Tkachuk, S. O. (2023). THE CONTENT OF INTERLEUKIN-1β, PROCALCITONIN IN THE SERUM OF PATIENTS WITH LACTATIONAL MASTITIS. Medical and Clinical Chemistry, (2), 51–55. https://doi.org/10.11603/mcch.2410-681X.2023.i2.13972

Issue

No. 2 (2023)

Section

ORIGINAL INVESTIGATIONS

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