THE ANTIOXIDANT PROTECTION SYSTEM STATE OF RATS UNDER EXPERIMENTAL DOXORUBICIN INTOXICATION AND THE EFFECTS OF CORRECTING FACTORS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i1.13714Keywords:
antioxidant system, doxorubicin, intoxication, free radical oxidation, phosphodiesterase-3 inhibitor, ethylmethylhydroxypyridine succinateAbstract
Introduction. The doxorubicin model was used for research, which, according to many authors, most adequately reproduces heart failure of varying degrees of severity. Doxorubicin is an antibiotic that has a cytostatic effect. The mechanism of its action consists of interaction with DNA, formation of free radicals, and inhibition of nucleic acid synthesis. The doxorubicin action and the development of hypoxia are accompanied by the activation of oxidative stress, the strengthening of free radical processes, and changes in the metabolism of carbohydrates, proteins, and lipids.
The aim of the study – the effect of the drug “Bendamine” on the indicators of antioxidant protection of the body of rats under experimental simulation of heart failure.
Research Methods. Modeling of heart failure was performed on sexually mature male Wistar rats with a body weight of 180–200 g. In blood and myocardial homogenate, the level of lipid peroxidation products, the activity of antioxidant enzymes, and the level of reduced glutathione were determined against the background of doxorubicin intoxication and when using the drug “Bendamine”.
Results and Discussion. During doxorubicin intoxication, LPO processes are enhanced in rats, which is indicated by the increased lipid hydroperoxides, diene conjugates, and TBA-active products in the blood and the homogenate of the rat myocardium of the first experimental group. It is worth noting that the inhibition of the antioxidant defense system was also established in intoxicated rats, as indicated by the low level of both enzymatic and non-enzymatic links of the antioxidant system. It was established that bendamine has a corrective effect on rats' oxidative stress conditions inherent in doxorubicin-induced heart failure. The drug “Bendamine” inhibits the excessive formation of POL products in pathologically changed tissues of the heart of rats and has an inducing effect on the system of antioxidant protection.
Conclusions. The influence of the drug "Bendamine" on the indicators of antioxidant protection of the body of rats under experimental simulation of heart failure was studied. Its antioxidant properties have been proven.
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