FEATURES OF ANTIOXIDANT PROTECTION IN THE SYNOVIAL MEMBRANE OF THE KNEE JOINT AT DIABETIC ARTHROPATHY
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i1.13712Keywords:
knee joint, diabetic arthropathy, synovial membrane, antioxidant protectionAbstract
Introduction. Joint damage in patients with diabetes is a fairly frequent complication. Features of changes in antioxidant protection in the synovial membrane of the knee joint in diabetic arthropathy have not been studied enough.
The aim of the study – to investigate the features of changes in antioxidant protection in the synovial membrane of the knee joint at diabetic arthropathy.
Research Methods. The synovial membrane of knee joint of 90 laboratory sexually mature white male rats, which were divided into 3 groups, was studied using a complex of biochemical and morphological methods. Group 1 included 30 intact, practically healthy animals, group 2 – 30 rats with one-month diabetic arthropathy of the knee joint, group 3 – 30 animals with the two-month specified pathology. Diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin manufactured by “Sigma” at a dose of 50 mg/kg. Rats were euthanized by bloodletting under thiopental anesthesia. Indicators of antioxidant protection were determined in the synovial membrane of animals. The relative volumes of damaged venous and arterial endotheliocytes and synoviocytes were determined on histological preparations of synovial membrane. Quantitative indicators were processed statistically.
Results and Discussion. It has been established that with diabetic arthropathy of the knee joint, the antioxidant protection of the synovial membrane significantly deteriorates. With one-month diabetic damage to the knee joint, the activity of superoxide dismutase in the synovial membrane decreased by 12.2 %, catalase by 13.8 %, and ceruloplasmin by 8.5 % (p<0.001); with two-month diabetic atropopathy, these indicators decreased by 18.3 %, 20.0 %, and 19.2 %, respectively (р<0.001). The relative volume of damaged endotheliocytes in the arteries of the synovial membrane increased by 10.7 times, in the veins by 12.1 times, and synovial cells by 10.4 times (p<0.001), in two-month diabetic arthropathy of the knee joint, the given indicators have similarly changed in 15.6; 17.7 and 14.6 (p<0.001) times.
Conclusions. Diabetic arthropathy of knee joint is characterized by a significant decrease in antioxidant protection of synovial membrane of the knee joint, apoptosis, dystrophy, necrobiosis of synovial cells, endotheliocytes, stroma growth, and focal infiltrates. The most pronounced changes in indicators of antioxidant protection and damaged endotheliocytes in arterial and venous channels and affected synoviocytes of the synovial membrane were found in two-month diabetic arthropathy of the knee joint.
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