LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN WHITE RATS CONSUMING DRINKING WATER WITH DIFFERENT CONTENTS OF SODIUM MONOPHOSPHATE
DOI:
https://doi.org/10.11603/mcch.2410-681X.2023.i1.13583Keywords:
lipid peroxidation, antioxidant protection, phosphates, drinking waterAbstract
Introduction. The problem of anthropogenic pollution of surface water bodies with phosphates is one of the causes of eutrophication of water bodies, which leads to the accumulation of biotoxins, the deterioration of water quality, and negatively affects the physiological and biochemical processes of hydrobionts and harms human health. The starting mechanism of pathological processes in the body are membrane-damaging processes, among which lipid peroxidation (LPO) occupies a significant place.
The aim of the study – to establish the peculiarities of the course of LPO processes and the state of antioxidant protection (AOP) in the liver homogenate of white rats when drinking water with different concentrations of phosphates is used for 30 days.
Research Methods. Experiments were conducted on 36 white purebred male rats weighing 180–200 g, divided into 6 groups, which consumed water with the addition of sodium monophosphate in doses of 0.01; 0.1; 1.0; 10.0; 100.0 mg/dm3 in terms of elemental phosphorus. The content of TBA active products and diene conjugates (DK) was determined in the liver homogenate, and the state of AOP was assessed by the activity of superoxide dismutase (SOD) and catalase.
Results and Discussion. The obtained data indicate that concentrations of sodium monophosphate in drinking water at the level of 0.01, 0.1 and 1.0 mg/dm3 did not have a pronounced effect on the processes of POL in the body of rats. However, phosphates at the level of 10.0 and 100.0 mg/dm3 cause an increase in the content of TBK-AP and DC in the 4th group – by 90 and 73 % and in the 5th – by 92 % and 75 % (p<0, 05) respectively. It was also established that the activity of SOD decreased statistically significantly only in the 5th experimental group – by 45 % (p<0.05) and the activity of catalase increased statistically significantly in the 5th and 4th groups – by 56.5 % (p<0.05) and 34.2 % (p<0.1), respectively.
Conclusions. Drinking water with phosphates concentrations of 10.0 and 100.0 mg/dm3 has a negative effect on the state of cell membranes of hepatocytes due to the activation of POL processes. The detected changes in the body of experimental rats can be a specific pathogenic link in the formation of pathological changes in the body under simulated conditions, and the disruption of the AOP as a result of such an effect contributes to the strengthening of the destruction of membranes and the violation of their structural and functional properties.
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