CHANGES IN THE CONTENT OF MIDDLE WEIGHT MOLECULES IN FEMALE RATS WITH OVARIAN PATHOLOGY AND THEIR CORRECTION BY INOSITOL
DOI:
https://doi.org/10.11603/mcch.2410-681X.2022.i4.13579Keywords:
medium-weight molecules, ovaries, inositol, FT 500 plusAbstract
Introduction. The problem of infertility is still relevant today. The main reasons for its development are: age; stress, sedentary lifestyle, exposure to heavy metal salts. Inositol preparations (In) improve a woman's reproductive function.
The aim of the study – to assess the development of endogenous intoxication in female rats with ovarian pathology and to carry out correction with In preparations.
Research Methods. Experiments were performed on 144 white female Wistar rats, which were divided into 4 series: 1 – control, 3.5-4-month-old rats, 2 – rats of 7.5–8 months, 3 – rats that were intragastrically injected with lead acetate solution at a dose of 0.05 mg/kg for 15 days , 4 – hypodynamic stress. There were 3 groups in each series: A – control (C), B – In, B – FT 500 plus (FT). In and FT was administered intragastrically for 15 days at a dose of 400 mg/kg of In. Middle weight molecules (MWM) were determined in blood serum at λ 280 nm, 260, 254, and 238 nm.
Results and Discussion. In the control, MWM238 were highest in 3A. In 2A and 4A, the values were lower than K by 35.7 % and 67.9 %. MWM254 increased in 2A and 3A, compared to 1A, by 2.4 times and 3.0 times. MWM260 were greater than the indicators of 1A only in the 2nd series by 44.4 %. MWM280 were the smallest in the K series of rats. Yin in rats of the 1st series caused a decrease in MWM238 and MWM280, and an increase in MWM254 and MWM260. FT resulted in a decrease in MWM238 but an increase in MWM254 and MWM280. In series 2, In contributed to the reduction of MWM254 and MWM280. FT resulted in a decrease in all MWM. In series 3, Ying and FT contributed to the decrease of MWM238 and MWM280, and the growth of MWM260. In series 4, In caused a decrease in MWM280 but an increase in MWM254. FT resulted in increased MSM238, MWM254 and MWM280.
Conclusion. In preparations at a dose of 400 mg/kg should not be used in an experiment for healthy young female rats. In 7.5–8 month old animals, FT is optimal for reducing endogenous intoxication. In animals exposed to nanodoses of lead acetate, the effectiveness of using inositol and FT is the same. The use of inositol preparations is not effective in animals that have been in hypodynamia and stress for a long time.
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