ANALYTICAL CONTROL OF CEREAL CROP PROTECTION SYSTEM PESTICIDE RESIDUES IN THE WATER AND AIR
Keywords:chromatographic analysis, high performance liquid chromatography, pesticides, cereals
Introduction. Chromatographic analysis to develop conditions for determining 9 active substances (prosulfuron and pinoxaden herbicides, thiamethoxam and lambda-cyhalothrin insecticides, azoxystrobin, benzovindiflupyr, cyproconazole, propiconazole fungicides, trinexapac-ethyl plant growth regulator) of the cereal crop protection system in their combined presence was performed using analytical standards from which the initial standard solutions of each of the test compounds were prepared in acetonitrile. Chromatographic analysis of each calibration solution of the mixture was performed 3 times to construct the graphs of dependence between the area of the compound chromatographic peak and its concentration. Quantitative determination of the test compound was performed by the method of absolute calibration (external standard method) and the obtained results were processed by statistical methods.
The aim of the study – development and improvement of analytical control of residual amounts of pesticides in water and air when it used as a part of grain protection system.
Research Methods. The findings allowed us to calculate the retention factor (k) and to construct dependences of relative retention of test compounds on columns C18 and CN on the content of acetonitrile in the mobile phase acetonitrile + 0.1 % aqueous solution of orthophosphoric acid and to continue research on compounds separation in its combined presence as more effective using column 250/4.6 Nucleosil 100-5 C18 and a mixture of acetonitrile + 0.1 % aqueous solution of orthophosphoric acid. According to the results of studying the absorption spectra, in order to improve the chromatograms of water samples and, more particular, air, the wavelength of the UV detector was optimized, which gave a positive result.
Results and Discussion. Conditions for water and air samples preparation for further chromatographic determination of test compounds and optimal conditions for extraction and chromatographic determination of different chemical class pesticides used in the cereal crops protection system. These conditions allow to control pesticides shared content in the water and air sample with limits of detection – 0.001 mg/dm3 and 0.05 mg/m3, respectively i.e., will allow controlling the approved hygienic standards of these compounds in the water and working zone air.
Conclusion. Developed conditions for determining 9 cereal crop protection system pesticides by high-performance liquid chromatography method in its shared presence in water and air samples, can significantly speed up the analysis, reduce costs for analysis performance and improve pesticides monitoring in the environment.
Tribel, C.O., Strigun, O.O., & Gamanova, O.M. (2016). Measures to protect against beetles. Quarantine and plant protection. (8-9), 19-23. Retrieved from: http://nbuv.gov.ua/UJRN/Kizr_2016_8-9_9 [in Ukrainian].
Ivashchenko, OO (2016). Realities and prospects of crop protection systems. Quarantine and plant protection, (11-12), 1-3. Retrieved from: http://nbuv.gov.ua/UJRN/Kizr_2016_11-12_3 [in Ukrainian].
Allowable doses, concentrations, quantities and levels of pesticides in agricultural raw materials, foodstuffs, air of the working zone, atmospheric air, water of reservoirs, soil, 244 pages. State Sanitary Rules and Norms 18.104.22.168.3.4.-000-2001 § Ministry of Health of Ukraine (2001). Retrieved from https://zakon.rada.gov.ua/rada/show/v0137588-01#Text [in Ukrainian].
On the approval of Hygienic standards and regulations of safe use of pesticides and agrochemicals, No. 55 with amendments Order § Ministry of Health of Ukraine (2016). Retrieved from https://zakon.rada.gov.ua/laws/show/z0207-16#Text [in Ukrainian].
Kudris, I.V., & Kulikov, A.Yu. (2014). Evaluation of the variability of relative retention times when using chromatographic columns with grafted C18 groups. Methods and Objects of Chemical Analysis, (9, No. 1), 12-18. Retrieved from http://nbuv.gov.ua/UJRN/Moca_2014_9_1_4 [in Russian].
Sirotchuk, O.A., Didukh, I.R., Kuras, S.F., & Zaitsev, V.M. (2015). Chromatographic separation of anti-cold components using stationary phases containing incorporated polar insert. Methods and objects of chemical analysis, (10, № 4), 171-177. Retrieved from http://nbuv.gov.ua/UJRN/Moca_2015_10_4_4 [in Ukrainian].
Klisenko, M.A., Alexandrova, L.G., Demchenko, V.F., & Makarchuk, T.L. (1999). Analytical chemistry of pesticide residues: Textbook. manual. Kyiv: ECOGINTOX [in Ukrainian].
ISO 8466-1:1990. Water quality – Calibration and evaluation of analytical methods and estimation of performance characteristics – Part 1: Statistical evaluation of the linear calibration function [in Russian].
(1999). On the use of norms of accuracy and correctness of measurements in the control of chemicals in food raw materials, food and environmental objects and the correspondence between the values of MAR and MAC and the limits of analytical determination of chemicals. April 20, 1999 Resolution No. 20 § Ministry of Health of Ukraine [in Ukrainian].
Bublik, L.I., & Gavrilyuk, L.L. (2014). Methods of monitoring and control of pesticide residues in agrocenoses. Plant Protection and Quarantine, (60), 53-66. Retrieved from: http://nbuv.gov.ua/UJRN/Zikr_2014_60_9 [in Ukrainian].
How to Cite
Copyright (c) 2023 Medical and Clinical Chemistry
This work is licensed under a Creative Commons Attribution 4.0 International License.