THE EFFECT OF L-ARGININE AND AMINOGUANIDINE ON THE DEVELOPMENT OF OXIDATIVE STRESS IN THE LUNGS IN EXPERIMENTAL ANTIPHOSPHOLIPID SYNDROME
Keywords:antiphospholipid syndrome, oxidative stress, L-arginine, aminoguanidine
Introduction. Antiphospholipid syndrome is an autoimmune disease accompanied by the formation of antiphospholipid antibodies which entering into blood coagulation reactions, cause thrombosis of veins and arteries, as well as contribute to miscarriage. At the same time, the processes of free radical oxidation are activated, which is a consequence of ischemia, the antioxidant system is depleted, which deepens lung damage.
The aim of the study – to investigate the state of the prooxidant-antioxidant system in the lung tissue with experimental APS and when using modulators of nitric oxide synthesis (L-arginine and aminoguanidine).
Research Methods. Modeling of the antiphospholipid syndrome was performed on female BALB/c mice. The level of oxidation products and the activity of antioxidant enzymes were determined under APS and with the use of L-arginine and aminoguanidine in the lung tissue.
Results and Discussion. Under conditions of experimental APS in the lungs of mice, an increase in the content of lipid hydroperoxides and TBA-active products, inhibition of the activity of superoxide dismutase and catalase, depletion pool of the reduced glutathione, and impaired function of respiratory chain enzymes – succinate dehydrogenase and cytochrome oxidase. Positive dynamics was noted in restoring the activity of the antioxidant system and blocking the destructive action of free radicals on the lung tissue with the introduction of L-arginine and aminoguanidine and their combination.
Conclusions. Thrombosis and ischemia that occur during APS disrupt the balance of the prooxidant-antioxidant system, increasing the formation of reactive oxygen species and suppressing protective functions. The combined use of L-arginine and aminoguanidine in antiphospholipid syndrome helps to reduce the manifestations of oxidative stress in the lungs, in particular, to reduce the level of lipid peroxidation, restore the activity and content of the components of the antioxidant system, which is accompanied by the restoration of the activity of respiratory chain enzymes.
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