DEVELOPMENT OF OXIDATIVE PROCESSES IN THE HEART OF RATS DURING THE DEVELOPMENT OF IMMOBILIZATION STRESS
Keywords:lipid peroxidation, antioxidant system, heart, immobilization stress, male rats
Introduction. In recent months, due to the war on the territory of Ukraine, the number of people who spent a long time in a sedentary position in a stressful state has increased significantly. The leading link in the development of any pathology remains the development of oxidative stress, which affects the development of disorders of the cardiovascular system.
The aim of the study – to evaluate the development of oxidative stress in the homogenate of the rat heart during the development of immobilization stress.
Research Methods. Experiments were performed on 60 outbred male rats weighing 180–210 grams. Animals were divided into 5 groups – control, stress 1 day, stress 2 days, stress 3 days, stress 4 days. Stress was modeled by one-hour immobilization of animals lying on the back. Animals in each group were investigated in control, 1 day after the last immobilization. Under thiopental-sodium anesthesia, heart sampling was performed, in the homogenate of which diene conjugates (DC), TBA-active products (TBA-ap), superoxide dismutase (SOD) and catalase activity (Cat) were determined.
Results and Discussion. In male rats, one day after a single episode of stress, compared to the control, an increase in the concentration of DC by 41.4 % (p<0.001) and TBA-ap – by 21.1 % (p<0.01) and an increase in SOD activity by 38.5 % (p<0.001) and catalase activity by 68.5 % (p<0.001) was noted. There was an increase in DC and TBA-ap after 2 and 3 days of stress, but the indicators decreased after 4 immobilizations. There was an increase in SOD and Cat after 2 days of stress, but the indicators decreased after 3 days, and after 4 days, compared to the previous term, it increased.
Conclusion. The development of oxidative stress in the heart of rats under immobilization stress depends on the number of stressful episodes. With three one-hour immobilization, distress develops in male rats, with one and two immobilization – eustress.
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