CHANGES IN ENDOGENOUS INTOXICATION INDICATORS IN THE BLOOD AND LUNGS OF RATS IN THE DYNAMICS OF THE CRUSH SYNDROME DEVELOPMENT
DOI:
https://doi.org/10.11603/mcch.2410-681X.2022.i3.13368Keywords:
traum, crush syndrome, endotoxicosis, lungs, ratsAbstract
Introduction. Crush syndrome (CS) is 15–24 % in the structure of traumatic lesions. One of the important links in the mechanisms of CS is the development of endogenous intoxication, the integral indicator of which is the content of medium-weight molecules (MWM). Metabolic disturbances in tissues subjected to compression and toxic products formed in the focus of compression lead to damage to vital organs, primarily the liver, kidneys and lungs.
The aim of the study – to investigate the dynamics of changes in the content of MWM in the blood serum and lung homogenate of rats on the model of endotoxicosis, which is formed during CS.
Research Methods. The experiments were carried out on 40 outbred mature white male rats weighing 180–200 g. The experimental model was a pathological process that developed in animals as a result of compression of the soft tissues of the left pelvic limb for 4 hours in a special device, constructed at the Department of Functional and Laboratory Diagnostics of I. Horbachevsky Ternopil National Medical University. The compressive surface area was 4 cm2, and the compression force was 4.25 kg/cm2. Animals were taken out of the experiment under thiopental anesthesia on the 1st, 3rd, 7th and 14th days of observation by heart puncture. Determination of MWM content was carried out according to the method of R.I. Lifshits.
Results and Discussion. In the dynamics of the post-traumatic period of CS, an increase in the level of endogenous toxins was established, which indicates a significant increase in the content of MWM on the entire day of observation vs. control. The maximum values of the studied parameters were observed on the 3rd day of the experiment: in the blood serum – an increase in the content of MWM1 and MWM2 by 2.1 and 2.4 times, respectively; in the supernatant of the lung homogenate – an increase in the content of MWM1 and MWM2 by 2.2 and 2.8 times, respectively.
Conclusions. Experimental CS is accompanied by an increase in the level of endogenous intoxication, reaching a maximum on the 3rd day of the post-traumatic period. When comparing the dynamics of MWM changes, their synchronous increase was established both in blood serum and in the lung homogenate with more intense changes in the lung homogenate, which is probably associated with destructive changes, the development of inflammation, hypoxia, a decrease in the energy potential of the lungs and activation of lipids peroxidation.
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