THE ROLE OF LIPID PEROXIDATION PROCESSES IN THE HEART OF RATS OF DIFFERENT SEXES IN THE DYNAMICS OF THE DEVELOPMENT OF CRANIOCEREBRAL INJURY

Authors

  • O. V. Denefil I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • M. I. Medynskyі I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • Z. V. Salii I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • M. I. Kulitska I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • I. Ya. Andriichuk I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • R. S. Usynskyi I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2022.i3.13361

Keywords:

lipid peroxidation, antioxidant system, heart, craniocerebral trauma, rats of different sexes

Abstract

Introduction. Recently, the number of people with polytrauma has increased significantly, but craniocerebral injury remains quite common. Men with head injuries are characterized by a higher risk in all clinical and nosological groups compared to women. The leading link in the development of pathology remains oxidant stress, which affects various organs and systems, including the cardiovascular system.

The aim of the study – to evaluate the development of oxidative stress in the heart homogenate of rats of different sexes in different periods of development of craniocerebral injury.

Research Methods. Experiments were performed on 80 outbred rats of different sexes weighing 190–240 grams. Animals were divided into 2 groups – control and craniocerebral injury. Trauma was performed in animals under sodium thiopental anesthesia by a single dosed blow to the skull with an energy of 0.375 J at a point located 5 mm in front of the interauricular line. Slaughter of animals was carried out in control, 1, 7, 14 and 28 days after injury. A heart sample was taken, in the homogenate of which diene conjugates (DC), TBA-active products (TBA-ap), superoxide dismutase (SOD) and catalase activity (Cat) were determined. A morphological study of the myocardium was carried out in Heidenhain-stained preparations.

Results and Discussion. In control rats-males, compared to females, DC prevailed, but superoxide dismutase and catalase activities were lower. During the experiment, an increase in DC and TBA-ap indicators was noted to a greater extent in males, while SOD and Cat activity remained higher in females. By the end of the experiment, there was no recovery of the studied indicators.

Conclusion. The development of oxidative stress in the heart of rats with craniocerebral trauma depends on the initial activity of the antioxidant system and gender. More pronounced damage was noted in rats-males. Greater activity of antioxidants prevents significant damage to the myocardium.

References

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Published

2022-12-16

How to Cite

Denefil, O. V., Medynskyі M. I., Salii, Z. V., Kulitska, M. I., Andriichuk, I. Y., & Usynskyi, R. S. (2022). THE ROLE OF LIPID PEROXIDATION PROCESSES IN THE HEART OF RATS OF DIFFERENT SEXES IN THE DYNAMICS OF THE DEVELOPMENT OF CRANIOCEREBRAL INJURY. Medical and Clinical Chemistry, (3), 27–32. https://doi.org/10.11603/mcch.2410-681X.2022.i3.13361

Issue

Section

ORIGINAL INVESTIGATIONS