PATHOGENETIC FEATURES OF THE FUNCTIONING OF THE ARGINASE/ NO-SYNTHASE SYSTEM IN SEMINAL PLASMA OF MEN WITH IDIOPATHIC INFERTILITY
DOI:
https://doi.org/10.11603/mcch.2410-681X.2022.i3.13357Keywords:
idiopathic infertility, seminal plasma, arginase, cNO-synthase, iNO-synthase, malondialdehydeAbstract
Introduction. The specific gravity of men with infertility is constantly increasing both in Ukraine and throughout the world. In the absence of reliable etiological factors of its occurrence, infertility is identified as idiopathic. It occurs in 25–30 % of men. As for pathochemical studies, it is assumed that in many cases idiopathic male infertility is caused by oxidative stress. However, it is also known that an important regulatory role in maintaining cellular homeostasis belongs to nitric oxide as a secondary messenger - a universal regulator of almost all physiological functions of the body.
The aim of the study – to find out the functional activity of the enzymes of the arginase/NO-synthase system and to identify new potential markers of idiopathic male infertility.
Research Methods. The study was conducted on the seminal plasma of 33 infertile men with idiopathic infertility aged 22–48. The control group included 27 fertile healthy men of the same age. The activity of such enzymes as arginase, constitutive and inducible isoforms of NO-synthase, as well as concentrations of nitrate and nitrite anions and malondialdehyde were studied in seminal plasma.
Results and Discussion. It was found that with idiopathic male infertility, arginase activity in seminal plasma decreases by 1.6 times. At the same time, cNOS activity decreased by 1.3 times and iNOS activity increased by 24.6 times. The detected decrease in the arginase/NOS ratio by 3.1 times indicates a decrease in the activity of non-oxidative, arginase metabolism, which competes with the oxidative NO-synthase metabolism of L-arginine. A 25.7-fold decrease in the ratio of arginase/iNOS activity in seminal plasma can be an important indicator of the development of idiopathic infertility. When evaluating the intensity of functioning of the NO-synthase pathway of L‑arginine metabolism in idiopathic infertility, a probable increase in seminal plasma NO3- by 1.4 times and a decrease in NO2- by 1.5 times were found. A direct correlation between MDA concentrations and stable metabolites of NO – nitrate (NO3-) anions was revealed. The demonstrated direct correlation between iNOS activity and MDA concentration in seminal plasma (r=0.87) indicates that iNOS can activate lipid peroxidation processes in idiopathic infertility.
Conclusions. The development of idiopathic male infertility is accompanied by changes in the activity of enzymes of the arginine/NO-synthase system – a decrease in the activity of arginase and cNOS and an increase in the activity of iNOS, which indicates the dominance of the oxidative pathway of L-arginine metabolism. A decrease in the ratio of arginase/iNOS activity in seminal plasma can be an important indicator of the development of idiopathic infertility.
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