FEATURES OF DISORDERS ENERGY SUPPLYING OF THE MUSCLE OF LARGE INTESTINE AT CONDITIONS OF POSTRESECTION PORTAL HYPERTENSION

Authors

  • M. S. Hnatiuk I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • O. M. Protcailo I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • L. V. Tatarchuk I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • N. Ya. Monastyrska I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY
  • S. O. Konovalenko I. HORBACHEVSKY TERNOPIL NATIONAL MEDICAL UNIVERSITY

DOI:

https://doi.org/10.11603/mcch.2410-681X.2022.i2.13203

Keywords:

postresection portal hypertension, large intestine, muscle, energy supply

Abstract

Introduction. Postresection portal hypertension is complicated by plethora, lesions, deterioration of energy supply of the portal vein, which has not been studied in muscular membrane of the intestine.

The aim of the study – to establish the features of disorders of energy supply of muscular membrane of the colon in conditions of postresection portal hypertension.

Research Methods. The colon of 50 rats, which were divided into 3 groups, was biochemically and morphologically studied. Group 1 – 15 intact animals, 2 – 20 rats with postresection portal hypertension, 3 – 15 animals with a combination of postresection portal hypertension with multiorgan failure. Postresection portal hypertension was modeled by resection of left and right lateral lobes of the liver (58.1 % of its parenchyma). Rats were euthanized by bloodletting under anesthesia one month after the start of the experiment. The energy supply of the muscular membrane of the colon was determined by the content of ATP, ADP, AMP, ATP/ADP ratio. The thickness of muscular membrane of the colon, the relative volume of myocytes, stroma, damaged myocytes, stromal-myocyte ratios, diameter of myocytes, their nuclei, nuclear-cytoplasmic ratios in myocytes were measured on micropreparations. Correlation analysis was performed between biochemical and morphometric parameters. Quantitative values were processed statistically.

Results and Discussion. Resection of 58.1 % of the liver parenchyma led to postresection portal hypertension, dilatation and plethora of the portal hepatic vein, mesenteric veins, venous bed of the small and large intestine, splenomegaly, ascites, rarely to the development of multiple organ failure. Dystrophy, necrobiosis of epitheliocytes, myocytes, endotheliocytes, stromal structures, infiltrative and sclerotic processes were found in the wall of the colon. In the muscular membrane, stromal-myocyte ratios increased by 19.4%, nuclear-cytoplasmic ratios in myocytes – by 6.7 %, the relative volume of damaged myocytes – by 7.2 times, with development of multiorgan failure – by 25.7%, 9.1 %, 16.3 times, respectively. The levels of ATP, ADP, AMP, ATP/ADP ratio decreased in the muscular membrane, its energy supply deteriorated.

Conclusions. Removal of left and right lateral lobes of the liver in laboratory adult white male rats leads to the development of postresection portal hypertension and severe damage and deterioration of energy supply of muscular membrane of the colon. The degree of disruption of energy supply of muscular membrane depends on the severity of damage to its structures and the combination of postresection portal hypertension with multiorgan failure.

References

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Published

2022-10-04

How to Cite

Hnatiuk, M. S., Protcailo, O. M., Tatarchuk, L. V., Monastyrska, N. Y., & Konovalenko, S. O. (2022). FEATURES OF DISORDERS ENERGY SUPPLYING OF THE MUSCLE OF LARGE INTESTINE AT CONDITIONS OF POSTRESECTION PORTAL HYPERTENSION. Medical and Clinical Chemistry, (2), 33–38. https://doi.org/10.11603/mcch.2410-681X.2022.i2.13203

Issue

No. 2 (2022)

Section

ORIGINAL INVESTIGATIONS

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