MECHANISMS OF ADRENAL MYOCARDIAL DAMAGE DEVELOPMENT IN RATS WITH DIFFERENT MOTOR ACTIVITY
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i4.12740Keywords:
lipid peroxidation, antioxidant system, heart, adrenaline, motor activityAbstract
Introduction. One of the reasons for the development of cardiovascular pathology is stress, which releases excessive amounts of catecholamines, which cause the development of pathology.
The aim of the study – to evaluate the development of oxidative stress in the heart homogenate of male rats with different motor activity in adrenaline myocardial damage (AMD).
Research Methods. The experiments were performed on purebred rats weighing 180–220 grams. Selection of animals for motor activity was carried out by the method of "open field". Experiment on animals was performed in control, 1 and 24 hours after injection of adrenaline, which determined diene conjugates (DC), TBA-active products (TBA-ap), oxidatively modified proteins (OMP), superoxide dismutase (SOD) and catalase activity (Cat). Conducted morphological examination of the myocardium in Heidenhain-stained preparations.
Results and Discussion. In the control of highly active males, compared with low-activity, dominated by products of lipid peroxidation (DC, TBA-ap) and OMP, as well as higher superoxide dismutase and catalase activity. At the beginning of the development of AMD in all animals increased the content of DC, TBA-ap, OMP, and they remained higher in highly active rats. The activity of antioxidants increased. Moreover, the difference in SOD between the two groups of animals was not detected, and Cat was higher in low-activity rats. The number of necrosis was higher in animals with high motor activity. In 24 hours of AMD further increase of DC, TBA-ap, OMP, in comparison with control and the previous term of research is noted. There was also a decrease in antioxidant activity, which did not differ from those of control animals, but only in males with low motor activity. In animals with low motor activity, the activity of antioxidants exceeded the control value, and it was more than an hour after the injection of adrenaline. Changes in the indexes indicating the development of damage were significantly greater in animals with high motor activity.
Conclusion. The development of adrenaline damage to the heart depends on the motor activity of the animal. A more pronounced difference was observed in the experiment. Greater antioxidant activity prevents significant myocardial damage.
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