DETECTION OF MILNACIPRAN BIOTRANSFORMATION PRODUCTS IN URINE UNDER TLC-SCREENING
DOI:
https://doi.org/10.11603/mcch.2410-681X.2021.i4.12730Keywords:
milnacipran, chemical-toxicological analysis, biofluids, metabolites, thin layer chromatography, color reactionsAbstract
Introduction. Thin layer chromatography screening is the most accessible type of screening procedure that is used in forensic toxicology. For the analytical diagnosis of drug poisoning by the TLC method, it is important to develop conditions for the detection of both native compounds and products of their biotransformation in the biological samples.
The aim of the study – to develop a method for isolating the antidepressant drug milnacipran from human urine in the presence of its biotransformation products and determine the conditions for their detection by thin layer chromatography, suitable for analytical diagnostics of thymoleptic intoxication.
Research Methods. The study was carried out with human urine samples collected after taking a single therapeutic dose of milnacipran. The urine was subjected to the acid hydrolysis and the antidepressant and its metabolites were extracted from the destructate with chloroform from an alkaline medium at pH 8–9. Concomitant endogenous admixtures were removed by extraction with diethyl ether from an acidic medium at pH 1. For the chromatographic study of the extracts, four mobile phases recommended by the International Association of Forensic Toxicologists for TLC screening of drugs, and Merk chromatographic plates were used. Colour reactions were carried out on pieces of chromatographic plates with a range of chromogenic reagents most common used in chemical-toxicological analysis. Metabolites were identified by electron impact mass spectrometry.
Results and Discussion. The parameters of the chromatographic mobility of the main (N-desethylmylnacipran) and minor (the structure could not be established) metabolites of milnacipran were determined, as well as the results of the reactions of their visualization with chromogenic reagents.
Conclusions. Isolation conditions for milnacipran and its biotransformation products from human urine have been proposed. The method of the detection of the native compound and milnacipran metabolites in the extracts from urine by TLC and colour reactions after taking a single therapeutic dose of the drug has been developed. The methods are recommended for use in the practice of forensic and clinical toxicology.
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