FEATURES OF THE FUNCTIONAL STATE OF THE LIVER IN RATS OF DIFFERENT AGE WITH MODELED SECONDHAND TOBACCO SMOKING COMBINED WITH ADMINISTRATION OF MONOSODIUM GLUTAMATE
Keywords:secondhand tobacco smoking, monosodium glutamate, functional state of the liver, biochemical markers
Introduction. Every year tobacco smoking kills more that 8 million people all over the world; 1.2 of them are secondhand smokers. Meanwhile, the characteristic feature of modern food technologies is the usage of food supplements which are not always safe for human health.
The aim of the study – to investigate the changes of the liver functional state in blood serum of rats of different age in case of secondhand tobacco smoking combined with administration of monosodium glutamate.
Research Methods. Experiments were conducted on 32 outbred mature white male-rats 180–200 g weight and 32 outbred immature white male-rats 60–80 g weight. Each group of animals was divided into four subgroups: I – control (n=8); ІІ – rats with modeled secondhand tobacco smoking (n=8); ІІІ – rats, which were injected with monosodium glutamate (n=8); IV – rats with modeled secondhand tobacco smoking combined with the monosodium glutamate injection (n=8). In the blood serum of rats, the content of the general bilirubin, the activity of alkaline phosphatase (ALP) and the activity of the alaninaminotransferase (ALT) were determined.
Results and Discussion. In case of secondhand tobacco smoking in the blood serum of mature rats, the content of the general bilirubin significantly decreased by 35.3 %, the ALP activity significantly increased by 38.3 %, the activity of ALT was significantly increased by 2.6 times, which indicated a violation of the functional state of the liver and the development of cytolysis and cholestasis. Secondhand tobacco smoking on the background of the use of monosodium glutamate was accompanied by a decrease in the content of the total bilirubin by 55.3 %; p<0.001 vs control group, which is 31.0 % (p=0.002) below this indicator with an isolated action of tobacco smoke; an increase in ALP activity by 2.1 times; p<0.001 vs control group, which is 53.2 % (p<0.001) exceeded the data with an isolated action of tobacco smoke; an increase in the activity of ALT by 4.5 times; p<0.001 vs control group, which is 68.8 % (p<0.001) exceeded the data with an isolated action of tobacco smoke. In immature rats, the intensity of changes of biochemical markers of the liver functional state exceeds the rates of mature rats.
Conclusions. The results indicate that monosodium glutamate enhances the toxic effect of tobacco smoke on the liver, which is manifested by more pronounced changes in the biochemical markers of its functional state in case of the combined effect of tobacco smoke and monosodium glutamate versus to the isolated tobacco smoke action. In the age aspect, more pronounced changes are observed in immature rats.
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