SIGNIFICANCE OF M-CHOLINOR RECEPTORS IN CHANGES OF OXIDATIVE PROCESSES IN THE DEVELOPMENT OF ADRENALINE MYOCARDIAL DAMAGE IN HIGH-AND LOW-RESISTANT TO HYPOXIC HYPOXIA RATS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.i4.11741Keywords:
hypoxia, resistance, lipid peroxidation, antioxidant system, heart, adrenaline, atropine, carbacholinAbstract
Introduction. It becomes necessary to study the individual reactivity of the body due to the development of hypoxia in most pathological conditions.
The aim of the study – to evaluate the role of cholinergic regulation of the pro- and antioxidant system in the heart homogenate of high- and low-resistance to hypoxia (HR, LR) male rats in adrenaline myocardial damage (AMD).
Research Methods. The experiments were performed on HR and LR male rats weighing 180–220 grams. AMD was induced with adrenaline (1 mg/kg), and experiments were performed 24 hours after injection. The effect on M-cholinoreceptors was carried out with carbacholine (0.4 μg/kg) and atropine sulfate (1 mg/kg). Diene conjugates (DC), TBA-active products (TBA-ap), superoxide dismutase activity (SOD), and vitamin E content (vit E) were determined in the heart.
Results and Discussion. In control (C) SOD, vit. E were higher in LR. At AMD DС in HR, TBA-ap in HR and LR, SOD – in HR increased. There was no difference between HR and LR. After injection of carbacholin, compared with C, increased TBA-ap, SOD, vit. E. When AMD on the background of carbacholin, TBA-ap in HR were smaller than after carbacholin, did not differ from C, and in LR remained higher C. SOD, vit. E in HR and LR exceeded the values of K and AMD, and in HR also carbacholin. There was no difference between HR and LR. After injection of atropine, compared with K, in HR increased vit. E, in LR – TBA-ap and vit. E. At AMD and atropine, were increased DC in HR and LR, TBA-ap in LR, more in LR. In HR TBA-ap were at the level of C.
Conclusions. The development of adrenaline damage to the heart depends on resistance to hypoxia and cholinergic regulation of the body. Stimulation of M-cholinoreceptors promotes the activation of the antioxidant system, limits the development of oxidative stress, and their blockade causes the implementation of oxidative mechanisms in LR rats, increases the difference in the course of AMD between HR and LR animals.
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