INDICATORS OF OXIDATIVE STRESS IN RATS WITH PARACETAMOL HEPATITIS AND CORRECTION WITH A DENSE EXTRACT FROM MAITAKE MUSHROOMS
DOI:
https://doi.org/10.11603/mcch.2410-681X.2020.v.i3.11534Keywords:
maitake mushrooms, paracetamol, hepatitis, thick extract, oxidative stress, antioxidant propertiesAbstract
Introduction. The article presents the results of a study of the activity of oxidative processes and the state of the defense systems of rats under conditions of their defeat with paracetamol and after correction with a thick extract from maitake mushrooms.
The aim of the study – to learn the effect of a thick extract of maitake mushrooms on the activity of lipid peroxidation and oxidative modification of proteins under the conditions of paracetamol hepatitis in rats.
Research Methods. The studies were carried out on the male white rats. The animals were divided into 10 groups, each of which included 6 animals. Acute hepatitis was modeled by intragastric injection of paracetamol at a dose of 1250 mg/kg once a day (for 2 days) as a suspension in a 2 % starch gel solution. Correction of toxic damage was carried out with a thick extract of maitake mushrooms, which was administered intragastrically 2 hours before the introduction of paracetamol and daily after the injury at a dose of 150 mg/kg of body weight. The comparison drug "Silibor" was administered according to the same scheme as the studied extract at a dose of 20 mg/kg of animal body weight. On the 3rd, 7th and 10th days from the beginning of the lesion, the rats were euthanized with sodium barbamil. Liver homogenate and blood serum were examined. The activity of free radical oxidation processes in paracetamol hepatitis and after the introduction of corrective drugs was assessed by the activity of superoxide dismutase, catalase, the content of TBA-AP and OMP products.
Results and Discussion. An increase in the activity of free radical oxidation processes after paracetamol damage to rats is evidenced by a decrease in the activity of superoxide dismutase and catalase, an increase in the content of TBA-active products, products of oxidative modification of neutral and basic proteins in the blood serum and liver of animals. The effectiveness of the use of a thick extract of maitake mushrooms and its positive effect on the activity of lipid peroxidation and oxidative modification of proteins under conditions of paracetamol hepatitis in rats was experimentally proved.
Conclusions. The use of the investigated extract of maitake mushrooms under conditions of simulated acute hepatitis in rats led to the normalization of lipid peroxidation, oxidative modification of proteins and antioxidant protection.
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