CHANGES IN THE LIPID PEROXIDATION SYSTEM IN EXPERIMENTAL ANIMALS AFTER COMBINED ABDOMINO-SKELETAL TRAUMA AND ISCHEMIA REPERFUSION OF THE LOWER EXTREMITIES

  • S. V. Garian UKRAINIAN SCIENTIFIC AND PRACTICAL CENTER OF EMERGENCY AND DISASTER MEDICINE, MINISTRY OF HEALTH OF UKRAINE, KYIV
Keywords: ischemia, reperfusion, tourniquet, experiment, combined trauma, diene conjugates, bleeding

Abstract

Introduction. The article presents the results of an experimental study that examined the effect of lower extremity reperfusion, after the applying of hemostatic plaits, on changes in serum lipid peroxidation in case of combined abdominal and skeletal trauma and massive external blood loss. The results of the study showed that reperfusion of the lower extremities in combination with the simulated traumas caused a significant activation of the LPO system within 21 days after trauma.

The aim of the study – to learn the effect of limb reperfusion in a model of combined abdominal-skeletal injury on changes in lipid peroxidation system.

Research methods. An experiment was conducted on 130 non-linear male rats weighing 190–220 grams. In the study groups, reperfusion of the lower extremities in combination with abdominal-skeletal trauma was simulated and the dynamics of changes in TBA-active products, diene conjugates up to 21 days after the traumatic period were analyzed. The likelihood of differences between the control group and the study group was evaluated using the non-parametric Mann – Whitney test.

Results and Discussion. After analyzing the dynamics of changes in diene conjugates and TBA-active LPO products in the study groups, we obtained results showing that the imposition of hemostatic tourniquet on both lower extremities of the experimental animals caused significant activation of the LPO system in the post-traumatic period. According to their dynamics, the activation of the LPO system in the group of experimental animals with skeletal trauma and the imposition of hemostatic harnesses and the group of experimental animals where the skeletal trauma was combined with closed trauma of the abdominal organs were similar. Thus, ischemia-reperfusion of the lower extremities can be considered as a component of combined trauma.

Conclusion. The imposition of hemostatic tourniquet on the two lower extremities, within two hours, causes a prolonged activation of the lipid peroxidation system, which is confirmed by statistically significant (p<0.05) increase in the content of diene conjugates and TBA-active products, with a maximum after 21 days.

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Published
2020-04-30
How to Cite
Garian, S. V. (2020). CHANGES IN THE LIPID PEROXIDATION SYSTEM IN EXPERIMENTAL ANIMALS AFTER COMBINED ABDOMINO-SKELETAL TRAUMA AND ISCHEMIA REPERFUSION OF THE LOWER EXTREMITIES. Medical and Clinical Chemistry, (1), 42-48. https://doi.org/10.11603/mcch.2410-681X.2020.v.i1.11055
Section
ORIGINAL INVESTIGATIONS