INVESTIGATION OF THE EFFECT OF ARTEMISIA L. HERB EXTRACTS ON THE PROGRESS OF THE TOXIC TETRACHLOROMETHANE LIVER DAMAGE
Introduction. Artemisia L. genus species are the promising sources for the manufacturing of drugs with hepatoprotective activity. They are used as appetizing, anthelmintic, bactericidal, choleretic and anti-inflammatory remedies. The pharmacological activity of Artemisia L. species is caused by the presence of different groups of biologically active substances.
The aim of the study – to learn the acute toxicity and the effect of Artemisia absinthium and Artemisia vulgaris water-alcohol extracts on the progress of the toxic tetrachloromethane liver damage.
Research Methods. The method of preclinical study of drugs safety was used to determine the acute toxicity. The investigation of hepatoprotective activity of Artemisia absinthium and Artemisia vulgaris extracts was performed using the model of acute tetrachloromethane hepatitis. Hepatoprotector of local manufacturer (“Silibor” tablets) was used as the reference drug.
Results and Discussion. It was found that intragastric administration of Artemisia absinthium and Artemisia vulgaris extracts at the dose of 6000 mg/kg does not lead to the death of animals. There were no changes in the integral, hematological, biochemical parameters and in the morphological structure of the internal organs of experimental animals. It allows to characterize the extracts at this dose as almost non-toxic ones (V toxicity class, LD50>5000 mg/kg) according to the toxicity classification of substances. The results of Artemisia absinthium and Artemisia vulgaris extracts study indicate that they show the distinct hepatoprotective activity in condition of acute toxic liver damage. They suppress peroxide destructive processes and reduce the evolution of cytolysis syndrome and their effects are no inferior rather than the effect of tablets “Silibor”.
Conclusions. The study of acute toxicity of Artemisia absinthium and Artemisia vulgaris extracts after their intragastric administration at the dose of 6000 mg/kg does not lead to the animals death. They were tidy and had the good appetite. The animals reacted adequately to sound and light stimulation. The processes of urination and defecation were unchanged. Breathing disorders and seizures were not observed. The investigated Artemisia absinthium and Artemisia vulgaris extracts demonstrate hepatoprotective activity in condition of acute toxic liver damage. This is proved by decreasing in the intensity of lipid peroxidation and in the toxicity of tetrachloromethane. Biochemical parameters of the animals’ blood and the liver homogenate became responded to the level of intact animals.
Korsun, V.F., Korsun, E.V., Ogrenich, N.A., & Sultanbekov, B.A. (2014). Fitoterapiya semeynogo vracha [Herbal medicine of family doctor]. Moscow [in Russian].
Bobkova, I.A., & Burianova, V.V. (2017). Farmakohnoziia [Pharmacognosy]. Kyiv: Medytsyna [in Ukrainian].
Stefanov, O.V. (2001). Doklinichni doslidzhennia likarskykh zasobiv (metodychni rekomendatsii) [Preclinical studiesof drugs (Guidelines)]. Kyiv: Avitsena [in Ukrainian].
Tymchyshyn, O.L., Kresiun, V.Y., & Hodovan, V.V. (2011). Vplyv medhermu na funktsionalnyi stan pechinky pry hostromu toksychnomu hepatyti [Influence of medgerma on the functional state of the liver in acute toxic hepatitis]. Intehratyvna Antropolohiia – Integrative Anthropology, 2 (18), 66-73 [in Ukrainian].
Assaf, M.M., Shchokina, K.H., & Drohovoz, S.M. (2012). Eksperymentalne vyvchennia vplyvu hustykh ekstraktiv lopukha velykoho na perebih modelnoho hepatytu u shchuriv [Experimental study of the effect of thick burdock extracts on the course of model hepatitis in rats]. Ukrainskyi biofarmatsevtychnyi zhurnal – Ukrainian Biopharmaceutical Journal, 4 (21), 44-48 [in Ukrainian].
Chan, Y.S., Cheng, L.N., Wu, J.H., Chan, E., Kwan Y.W., Lee, S.M., … Chan, S.W. (2011). A review of the pharmacological effects of Arctium lappa (burdock). Inflammopharmacology, 19 (5), 245-254.
Lin, S.C., Lin, C.H., Lin, C.C., Lin, Y.H., Chen, C.F., Chen, I.C. & Wang, L.Y. (2002). Hepatoprotective effects of Arctium lappa Linne on liver injuries induced by chronic ethanol consumption and potentiated by carbon tetrachloride. / J. Biomed. Sci., 9 (5), 401-409.
Koiro, O.O., Tovchyha, O.V., & Shtryhol, S.Yu. (2011). Eksperymentalne obhruntuvannia zastosuvannia ekstraktu yahlytsi zvychainoi pry spoluchnomu toksychnomu urazhenni pechinky ta nyrok [Experimental justification for the use of common juniper extract in the connective liver and kidney toxicity]. Ukrainskyi biofarmatsevtychnyi zhurnal – Ukrainian Biopharmaceutical Journal, 2 (13), 24-28 [in Ukrainian].
Derzhavna Farmakopeia Ukrainy (2015): v 3 t. Derzhavne pidpryiemstvo “Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv” [State Pharmacopoeia of Ukraine: in 3 volumes SE “Ukrainian Scientific Pharmacopoeial Centre of Drugs Quality”]. 2nd edition. Kharkiv: State enterprise “Ukrainian Scientific Pharmacopoeial Centre of Drugs Quality” [in Ukrainian].
Dobrelia, N.V., Strielkov, Ye.V., & Bukhtiarova, T.A. (2014). Rozvytok yevropeiskoho zakonodavstva v sferi vykorystannia tvaryn u naukovykh eksperymentakh [Development of European legislation in the field of using animals in scientific research]. Farmakolohiia ta likarska toksykolohiia – Pharmacology and Drug Toxicology, 2, 88- 91 [in Ukrainian].
Commission of the European Communities (1991). Council Directive of 18 December 1986 on the Lows, regulating the Application of Principles of Good Laboratory Practice and the Verification of their Applications for Tests on Chemical Substances (87/18/EEC). The Rules Governing Medicinal Products in the European Community.
Menshikov, V.V., Delektorskaya, L.N., & Zolotnitskaya, R.P. (1987). Laboratornye metody issledovaniya v klinike. Spravochnik [The methods of the laboratory research in the clinic. Reference book]. Moscow: Meditsina [in Russian].
Struk, O.A. (2017). Dotrymannia etychnykh norm pry provedenni farmakolohichnykh doslidzhen [Compliance with ethical standards in the pharmacological research]. Vashkevych, V.V. (Ed.). Hileia: naukovyi visnyk. Zbirnyk naukovykh prats. Hileia: a scientific bulletin. Collection of scientific works. Kyiv: Hileia [in Ukrainian].
Poznyakov, V.S., & Ivanov, N.G. (1979). Izmenenie funktsionalnogo sostoyaniya u krys pri vozdeystvii chetyrekhkhloristogo ugleroda [Changes in functional status in rats when exposed to carbon tetrachloride]. Toksikologiya novykh promyshlennykh khim. Veshhestv – Toxicology of new industrial chem. Substances. Moscow: Meditsina [in Russian].
Korobeynikova, E.N. (1989). Modyfikatsiia vyznachennia produktiv perekysnoho okysliuvannia lipidiv u reaktsii z tiobarbiturovoiu kyslotoiu [Modification for determination of the products of lipid peroxidation in reaction with thiobarbituric acid]. Laboratornoe delo – Laboratory Work, 7, 8-10 [in Ukrainian].
Khramov, V.A., & Listopad, G. (1973). Modifikatsiya metoda opredeleniya ornitina po Chinard i ee ispolzovanie dlya kolichestvennogo opredeleniya syvorotochnoy arginazy [Modification of the Chinard method for determining ornithine and its use for the quantification of serum arginase]. Laboratornoe delo – Laboratory Science, 591-592.