INVESTIGATION OF ANTI-EXUDATIVE ACTIVITY OF SALVIA OFFICINALIS LEAVES EXTRACTS
Introduction. Improving of the pharmacotherapy of inflammation is an urgent task of modern pharmaceutical and medical science.
The aim of the study – to investigate the anti-inflammatory activity of 11 different extracts from the leaves of Common Sage (CS) on the model of carrageenan edema.
Materials and Methods. The experiment was performed on white outbred male rats weighing 200–250 g. The extracts were administered intragastrically in a dose range of 10–100 mg/kg, an hour later, 1 ml of a 0.1 % carrageenan solution was injected into the hind limb. The volume of edema was evaluated every hour for 4 hours. The comparison drug is diclofenac sodium.
Results and Discussion. Anti-inflammatory activity of 9 of 11 extracts was found out. The extract obtained from decoction of CS (extract No. 1), the extract obtained by extraction with 50 % ethanol (extract No. 2), the phenolic extract of CS with the addition of L-lysine (extract No. 4), and the complex of hydroxycinnamic compounds of CS (extract No. 11) reduced inflammation in the course of both the alternative and exudative phases of inflammation by 31–100 % (p<0.05). Phenolic complex purified from amino acids and metal ions (extract No. 7), saponin complex (extract No. 8), total phenolic complex (extract No. 9) and flavonoid complex (extract No. 10) reduced inflammation only at the alteration phase (reduction of edema 26–67 %, p<0.05). The extract obtained by extraction with 96 % ethanol (extract No. 3) reduced edema only during the exudative phase. The decrease in exudation against its background was 73–90 % (p<0.05). Under these conditions, only the complex of phenolic compounds mixed with L-arginine (extract No. 5) and the polysaccharide complex from the leaves of CS (extract No. 6) did not exhibit anti-exudative activity.
Conclusions. The obtained data characterize the studied extracts from the leaves of CS (except for extracts No. 5 and 6) as effective anti-inflammatory drugs that can reduce the inflammatory process that develops along the cyclooxygenase pathway. All the studied substances were not inferior to the anti-inflammatory activity of diclofenac sodium. The obtained results determine the need for further experimental and clinical research of leaves of CS and agents based on its basis.
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