PECULIARITIES OF CHANGES THE CYTOCINE PROFILE AT DAMAGE OF THE TESTES BY RUBOMYCIN HYDROCHLORIDE
Introduction. Rubomycin is an antitumor antibiotic of the anthracycline series with pronounced cytostatic action, which, when introduced into the body in men, can lead to structural and functional changes in the testes.
The aim of the study – to investigate the features of changes in the cytokine profile at damage of the testes by rubomycin hydrochloride.
Research Methods. Morphometric methods investigated the structures of the testes of 30 laboratory adult white male rats. In the blood serum, the concentration of tumor necrosis factor (TNF-a), interleukin (IL-2), (IL-6), (IL-10) was determined by the enzyme immunoassay. Histostereometrically, at the testes micronutrients, nuclear-cytoplasmic relation in vascular endothelial cells, spermatogenic epitheliocytes, as well as relative volumes of damaged endothelial cells, spermatogenic epitheliocytes, and stromal-parenchymatous relation in organs were determined. Correlation analysis was performed between cytokine concentrations and histostereometric parameters studied to determine the correlation coefficient (r). Quantitative indicators were processed statistically.
Results and Discussion. It was found that when exposed to rubomycin hydrochloride on the body, the concentration of IL-2 in the serum increased by 22.0 %, IL-6 – by 45.2 %, IL-10 – by 34.5 %, TNF-α – by 12.5 % (p<0.001). The uneven, disproportionate increase in cytokines content in the serum in simulated pathology indicated an imbalance of the cytokine profile. Nuclear-cytoplasmic relations in vascular endothelial cells increased by 5.3 %, in spermatogenic epitheliocytes – by 7.4 % (p <0.01), which indicated a violation of structural cellular homeostasis. The relative volume of damaged endothelial cells increased by 13.5 times, and the spermatogenic epitheliocytes – 19.4 times (p<0.001), which indicated significant structural and functional changes of this organ. The presence of strong positive correlation relations between the relative volumes of damaged endothelial cells, spermatogenic epitheliocytes and concentrations of IL-2, IL-6, IL-10 (r=+0.82±0.03). The smaller correlation between the indicated histostereometric parameters of the testes and the serum TNF-a concentration (r=+0.65±0.02) were found to be less positive. Between the stromal-parenchymal relations in the testes of the 2nd group of observations and the content of cytokines in the serum, the correlation coefficient ranged from 0.52 to 0.64.
Conclusions. The introduction of rubomycin hydrochloride into the body results in pronounced damage to the testes and a significant imbalance of the cytokines IL-2, IL-6, IL-10, TNF-a in the serum. The degree of impaired cytokine profile in modeled pathology correlates with the severity of changes in structural homeostasis, relative volumes of damaged endothelial cells, spermatogenic epitheliocytes, stromal-parenchymal relations in the investigated organ.
Povorozniuk, M.V. (2012). Poshyrenist ta osnovni prychyny bezpliddia u cholovikiv [Prevalence and main causes of infertility in men]. Meditsynskiye aspekty zdorovya muzhchiny – Medical Aspects of Men's Health, 3 (5), 62-73 [in Ukrainian].
Hrytsuliak, B.V., Spaska, A.M., & Hrytsuliak, V.B. (2010). Orkhoepidydymit [Orhoepididymitis]. Ivano-Frankivsk: Prykarpatskyi natsionalnyi universytet [in Ukranian].
Burbela, A.T., Shabrov, A.V., & Denysenko, P.P. (2003). Sovremennye lekarstvennye sredstva [Modern medicines]. Moscow: Olma-Ptess [in Russian],
Konovalenko, S.O., Hnatiuk, M.S., Yasinovskyi, O.B., & Tatarchuk, L.V. (2019). Morphometric analysis of peculiarities of the testicular arteries remodeling under the influence of rubomycin hydrochloride. Polish Science Journal, 11, 77-83.
Borys, R.M. (2013). Porushennia tsytokinovoho profiliu u dynamitsi eksperymentalnoi kranio-skeletnoi travmy ta yoho korektsiia fetalnymy nervovymy klitynamy [Disruption of the cytokine profile in the dynamics of experimental cranio-skeletal injury and its correction by fetal nerve cells]. Aktualnye problemy transportnoy meditsyny – Actual Problems of Transport Medicine, 2 (32), 32-36 [in Ukrainian].
Horalskyi, L.P. Khomych, V.P., & Kononskyi, O.I. (2011). Osnovy histolohichnoi tekhniky i morfofunktsionalni metody doslidzhen u normi ta pry patolohii [Fundamentals of histological technique and morphofunctional methods of research in normal and pathology]. Zhytomyr: Polissia [in Ukranian].
Avtandilov, G.G. (2002). Osnovy kolichiestvennoy patologicheskoy anatomii [Basis of quantitative pathological anatomy]. Moscow: Meditsyna [in Russian].
Hnatiuk, M.S., Tatarchuk, L.V., & Yasinovskyi, O.B. (2016). Morfometrychna otsinka osoblyvostei remodeliuvannia struktur dvanadtsiatypaloi kyshky pry rezektsii riznykh obiemiv pechinky [Morphometric evaluation of features of remodeling of structures of a duodenum at resection of different volumes of a liver]. Naukovyi visnyk Uzhhorodskoho universytetu – Uzhhorod University Scientific Bulletin. Series “Medicine”, 1 (53), 92-95 [in Ukrainian].
Reznikov, O.H. (2003). Zahalni etychni pryntsypy eksperymentiv na tvarynakh [General ethical principles of animal experiments]. Endokrynolohiia – Еndocrinology, 8, 1, 142-145 [in Ukrainian].
Lapach, S.N., Gubenko, A.V., & Babych, P.N. (2001). Statisticheskiye metody v mediko-biologichieskikh issliedovaniyakh Excell [Statistical methods in biomedical research Excell]. Kyiv: Morion [in Ukrainian].
Lapovets, N.Ye. (2009). Riven prozapalnykh tsytokiniv v syrovattsi krovi khvorykh na abdominalnyi tuberkulioz [The level of proinflammatory cytokines in the serum of patients with abdominal tuberculosis]. Visnyk Ukrainskoi medychnoi stomatolohichnoi akademii. Aktualni problemy suchasnoi medytsyny – Bulletin of the Ukrainian Medical Dental Academy. Actual Problems of Modern Medicine, 9, 4 (28), 132-135 [in Ukrainian].