GENDER DIFFERENCE OF FREE-RADICAL PROCESSES IN THE MYOCARDIUM AT ADRENALINE DAMAGE ON THE BACKGROUND OF LIGHT DEPRIVATION
Introduction. Coronary heart disease is the leading cause of death and is more common in men. Stress is prevalent risk factors of heart damage. The balance of the oxidant/antioxidant systems is important marker of metabolism disorder im myocardium at stress. It is proved that melatonin as antioxidant reduces adrenalin-induced myocardium necrosis. But, the gender difference of cardioprotective effect of melatonin in conditions of impaired pineal gland has not been sufficiently studied.
The aim of the study – to explore gender differences of lipid peroxidation activity in the myocardium at adrenalin-induced damage on the background of light deprivation.
Research Methods. In the myocardium of the ventricles of male and female rats, the activity of lipid peroxidation (LPO) and antioxidant system (AOS) was determined in case of adrenaline damage. Myocardial necrosis was simulated by introducing adrenaline (0.5 mg/kg) into the muscle against the background of light deprivation (10 days of complete darkness, lighting 0.5–1 LX). The control group consisted of animals under conditions of a preserved regime of day/night cycle change (12 hours of lighting 500 LX/12 hours of darkness 0.5–1 LX).
Results and Discussion. The development of the necrotic process in the rat myocardium on the background of ten-day light deprivation was accompanied by less activation of LPO compared with the indices of the animals of the control group. This was confirmed by a lower content of LPO products in the ventricular myocardium. AOS activity under such conditions was greater than in animals of the control group, especially in females. This regularity testified to the positive effect of preliminary ten-day light deprivation on the development of the necrotic process in the myocardium, which can be explained by enhanced synthesis of melatonin, which is an antioxidant, and was also characteristic of rats of both sexes, but the cardioprotective effect of light deprivation was more significant in females.
Conclusion. Light deprivation helps protect the heart from adrenaline damage, as evidenced by the lower activity of lipid peroxidation and the greater activity of enzymes of the antioxidant system, especially in females.
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