RESEARCH ON TANNINS OF AGRIMONIA EUPATORIA L.
Introduction. In Ukraine there are four species of plants of the genus Agrimonia L. Of all species, the most common is Agrimonia eupatoria L. The plant has a wide range of pharmacological activity – hepatoprotective, choleretic, astringent, anti-inflammatory, antimicrobial, antiviral, diuretic, hypoglycaemic.
The aim of the study – to investigate the tannins in the agrimony herb, depending on the place of growth and determine the localization of tannins in fresh leaves by microchemical reactions.
Research Methods. Objects of study – water extracts of dried herb and fresh leaves of Agrimonia eupatoria. Determination of tannin content was performed by spectrophotometric method according to SPhU 2.0.1. The qualitative composition and quantitative content of the BAS from the tannin group in the test object were determined by high performance liquid chromatography (HPLC) using Agilent 1200 3D LC System Technologies (USA). Cross sections through the petiole and the central vein of the leaf were made for carrying out microchemical reactions. The sections were treated with a 1 % solution of ferric ammonium sulphate (III). Temporary micropreparations were examined in a light trinocular microscope XSP-146T company ULAB. Photos were taken using a Canon EOS 550.
Results and Discussion. The content of tannins in terms of pyrogallol in the agrimony herb ranges from 1.60 to 2.10 %, depending on the place of growth. HPLC in the agrimony herb identified the following components of tannins: two fragments of hydrolysed tannins, four simple catechins, one complex catechin. The most common substances, that were found in the agrimony herb are epicatechin and epigallocatechin. The results of microscopic studies have shown that by means of reaction with 1 % aqueous solution of ferric (III) ammonium sulphate, the localization of condensed and hydrolyzed tannins can be established on the cross section of the petiole and leaf.
Conclusions. The method of spectrophotometry has determined the quantitative content of tannins in the dried agrimony herb depending on the place of growth and ranges from 1.67 % to 2.10 %. The HPLC method revealed, identified and quantified the content of tannins in the agrimony herb. The localization of tannins in different places on the cross-sections of the petiole and leaf slices of the agrimony herb was determined.
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