EFFECT OF VANADIUM AND CHROMIUM CITRATE ON THE STATUS OF PRO-ANTIOXIDANT SYSTEM IN THE PANCREATIC TISSUES OF RATS WITH ALLOXAN DIABETES MELLITUS
Introduction. Diabetes increases oxidative stress, which causes exhaustion of the antioxidant system.
The aim of the study of the work is to investigate the influence of vanadium and chromium citrate on the antioxidant activity in the tissues of rats with alloxan diabetes mellitus. Vanadium and Chromium compounds are antioxidants, so they can be considered as a prophylactic agent to slow the progression of diabetes and the risk of complications.
Research Methods. The research was conducted on 32 laboratory rats of the Wistar line. The animals were divided into four groups. Rats from groups І (control) and ІІІ were given pure water without any additives; groups ІІ and ІV were given water with the solution of vanadium citrates, in the amounts of 0.5 μg V/ml of water, and chromium citrates – 0.1 μg Cr/ml of water. In the animals from groups III and IV, diabetes mellitus was induced by intraperitoneal injection of 5% solution of alloxan monohydrate in the amounts of 150 mg/kg of body weight. Materials for the research were homogenates of pancreas tissues of rats. We determined the content of lipid peroxidation products: lipid hydroperoxides and TBA-active products and the activity of antioxidant enzymes: superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and the content of reduced glutathione.
Results and Discussion. The combined action of vanadium citrate and chromium decreased the content of TBA-active products and increased the activity of antioxidant enzymes in intact animals. During experimental diabetes mellitus, the content of lipid peroxidation products increased, but there was a depletion of the protective capabilities of the antioxidant protection system. The prior introduction of vanadium and chromium citrates into the diet of rats with experimental diabetes contributed to the increase of the activity of the studied enzymes of the antioxidant system to the level of control. Obviously, trace elements of vanadium and chromium, as antioxidants, have the ability to be free radical scavengers and, accordingly, to reduce oxidative stress in diabetic animals.
Conclusions. During diabetes, hyperglycemia leads to an increase in lipid peroxidation products and a decrease in antioxidant protection. With the combined action of vanadium and chromium citrate, the indicators of the antioxidant system were normalized in the tissues of diabetic rats.
Praveena, S., Pasula, S., & Sameera, K. (2013). Trace elements in diabetes mellitus. Journal of Clinical and Diagnostic Research, 7 (9), 1863-1865. doi: 10.7860/JCDR/2013/5464.3335.
Zhao, L., Pu, L., Wei, J., Li, J., Wu, J., Xin, Z., Gao, W., & Guo, C. (2016). Brazilian green propolis improves antioxidant function in patients with type 2 diabetes mellitus. International Journal of Environmental Research and Public Health, 13 (5), 498. doi: 10.3390/ijerph13050498.
Ishida, T., Iizuka, M., Ou, Y., Morisawa, S., Hirata, A., Yagi, Y., & Miyamura, M. (2019). Juzentaihoto hot water extract alleviates muscle atrophy and improves motor function in streptozotocin-induced diabetic oxidative stress mice. Journal of Natural Medicines, 73 (1), 202-209. doi:10.1007/s11418-018-1269-8.
Pessoa, J.C., Etcheverry, S., & Gambino, D. (2015). Vanadium compounds in medicine. Coordination Chemistry Reviews, 301, 24-48. doi: 10.1016/j.ccr.2014.12.002.
Tripathi, D., Mani, V., & Pal, R. P. (2018). Vanadium in biosphere and its role in biological processes. Biological Trace Element Research, 186 (1), 52-67. doi: 10.1007/s12011-018-1289-y.
Bhuiyan, S., Shioda, N., Shibuya, M., Iwabuchi, Y., & Fukunaga, K. (2009). Activation of endothelial nitric oxide synthase by a vanadium compound ameliorates pressure overload-induced cardiac injury in ovariectomized rats. Hypertension, 53, 57-63. doi: 10.1161/HYPERTENSIONAHA.108.118356.
Feng, W., Mao, G., Li, Q., Wang, W., Chen, Y., Zhao, T., Li, F., Zou, Y., Wu, H., Yang, L., & Wu, X. (2015). Effects of chromium malate on glycometabolism, glycometabolism-related enzyme levels and lipid metabolism in type 2 diabetic rats: A dose-response and curative effects study. Journal of Diabetes Investigation, 6 (4), 396-407. doi: 10.1111/jdi.12350.
Vincent, J.B. (2015). Is the pharmacological mode of action of chromium (III) as a second messenger? Biological Trace Element Research, 166 (1), 7-12. doi: 10.1007/s12011-015-0231-9.
Lushchak, O.V., Kubrak, O.I., Torous, I.M., Nazarchuk, T.Yu., Storey, K.B., & Lushchak, V.I. (2009). Trivalent chromium induces oxidative stress in goldfish brain. Chemosphere, 75, 56-62.
Vlizlo, V., Iskra, R., Maksymovych, I., Lis, M.W., & Niedziolka, J.W. (2014). Disturbance of antioxidant protection and nature resistance factors in rats with different availabilities of trivalent chromium. Turkish Journal of Veterinary and Animals Sciences, 38 (2), 138-144.
Vincent, J.B. (2007). The nutritional biochemistry of chromium (III). Department of Chemistry the University of Alabama Tuscaloosa, USA.
Mironchik, V.V. (1998). Sposob opredeleniya gidroperekisey lipidov v biologicheskikh tkanyakh [Method of determining the content of lipid hydroperic acids in biological tissues]. Avtorskoye svidetelstvo №1084681 SSSR, MKI G №33/48. (SSSR). №3468369/2813; Byul. № 13. [in Russian].
Korobeynikova, S.N. (1989). Modifikatsiya opredeleniya produktov POL v reaktsii s tiobarbiturovoy kislotoy [Modification of definition of lipid peroxidation products in reaction with thiobarbituric acid]. Laboratornoye Delo – Laboratory Case, 7, 8-9 [in Russian].
Chevari, S., Andyal, T., & Shtrenger, Ya. (1991). Opredelenie antioksidantnykh parametrov krovi i ikh diagnosticheskoye znachenie v pozhilom vozraste [Determination of antioxidant parameters of blood and their diagnostic value in old age]. Laboratornoye Delo – Laboratory Case, 10, 9-13 [in Russian].
Moin, V.M. (1986). Prostoy i spetsificheskiy metod opredeleniya aktivnosti glutationperoksidazy v eritrotsitakh [A simple and specific method for the determination of glutathione peroxidase in erythrocytes]. Laboratornoye Delo – Laboratory Case, 12, 724-727 [in Russian].
Korolyuk, M.A., Ivanova, M.I., Mayorova, I.T., & Tokarev, V.E. (1988). Metod opredeleniya aktivnosti katalazy [Method for determination of catalase activity]. Laboratornoye Delo – Laboratory Case, 1, 16-19 [in Russian].
Bergmeyer, H.U., & Bernt, E. (1974). UV-assay with pyruvate and NADH. In Methods of Enzymatic Analysis (Second Edition), 2, 574-579.
Batler, O., & Dyubra, O. (1982). Metodika opredeleniya urovnya vostanovlenogo glutationa (GSN) v eritrotsitakh krovi (po printsipu Batler, O. Dyubon, B. Kelli, 1963): metodicheskie rekomendatsii po differentsialnoy diagnostike razlichnykh form ishemicheskoy bolezni serdtsa s ispolzovaniem opredeleniya komponentov glutationovoy protivoperekisnoy kataliticheskoy sistemy v eritrotsitakh krovi [Method for determining the level of restored glutathione (GSH) in erythrocytes of blood (on the principle of Butler, O. Dyubon, B. Kelly, 1963): guidelines for the differential diagnosis of various forms of coronary heart disease using the definition of the components of the glutathione antiperoxidative catalytic system in erythrocytes of the blood]. Odesa. [in Russian].