THE OXIDATION PROCESSES IN DIFFERENT AGE RATS UNDER THE CONDITIONS OF COMPLEX PERSISTENT EFFECT OF HEAVY METAL SALTS AND PHOSPHORORGANIC PESTICIDES

  • Ye. B. Dmukhalska I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
  • T. Ya. Yaroshenko I. HORBACHEVSKY TERNOPIL STATE MEDICAL UNIVERSITY
Keywords: oxidative stress, oxidation modification of proteins, lead acetate, copper sulfate, glyphosate, chronic effects, rats

Abstract

Introduction. Lead poisoning is a major public health risk. Lead is an immunotoxicant, it has reproductive toxicity, liver toxicity. Lead, copper and their compounds exhibit cumulative, cytotoxic effects on the body, cause the activation of oxidative stress.

 The aim of the study – to research the correction effect of the peptide on the oxidative processes in different age groups of animals by the lead acetate, the copper sulfate, the glyphosate (in herbicide Roundup) poisoning.

Research Methods. Experiments were performed on lab nonlinear white male rats of three age periods: puberty (young, 70–90 g of body weight and aged 2 to 3 months); mature (adult, weighing 170–210 g and aged 5–8 months) and old rats (body weight 250–300 g and aged 20–24 months), which were injected intragastrically for 30 days with the lead acetate, the copper sulfate, the glyphosate (in herbicide Roundup) and underwent the correction of violations of free radical oxidation of lipids and proteins with peptide cysteyl-histidyl-tyrosyl-histidyl-isoleucine. We studied free radical oxidation processes by the content of TBA-active products (TBA-AP), diene conjugates (DC), and oxidation-modified proteins in blood serum (OMP370 and OMP430).

Results and Discussion. The combined effect of lead acetate, copper sulfate and glyphosate on the oxidative modification of proteins and lipids in rats of different age groups was studied. It was established that the studied xenobiotics increase the concentration of TBA-active products, diene conjugates, and oxidative modification of proteins.

Conclusion. It was established that the effect of the lead acetate, the copper sulfate, the glyphosate (in herbicide Roundup) caused oxidative stress in rats, which was accompanied by an increase in the content of free radical oxidation products of lipids and proteins.

References

Ahamed, M., & Siddiqui, M.K.J. (2007). Envi­ronmental lead toxicity and nutritional factors. Environ­mental lead toxicity and nutritional factors. Clin. Nutr., 26 (4), 400-408.

Jadhav, S.H., Sarkar, S.N., Patil, R..D., & Tripa­thi, H.C. (2007). Effects of subchronic exposure via drinking water to a mixture of eight water contaminating metals: A biochemical and histopathological study in male rats. Arch. Environ. Con.. Toxicol., 53 (4), 667-677.

Vaziri, N.D., & Sica, D.A. (2004). Lead-induced hypertension: Role of oxidative stress. Curr. Hyper. Rep. J., 6, 314-320.

Baranowska-Bosiacka, I., Gutowska, I., Rybi­cka, M., Nowacki, P., & Chlubek, D. (2012). Neurotoxicity of lead. Hypothetical molecular mechanisms of synaptic function disorders. Neurol. Neurochir. Pol. J. 46(6), 569-578.

Omobowale T.O, Oyagbemi A.A, Olopade, J.O., Saba, A.B., & Akinrinde, A.S. (2014). Failure of recovery from lead induced hepatoxicity and disruption of erythro­cyte antioxidant defence system in wistar rats. Environ. Toxicol. Pharm., 37 (3), 1202-1211.

Barros, R.C.H., Bonagamba, L.G.H., & Machado, B.H. (2002) Cardiovascular responses to chemoreflex activation with pottassium cyanide or hypoxic hypoxia in awake rats. Autonomic Neuroscience: Basic, and Clinical, 97, 110-115.

Zakon Ukrainy “Pro zakhyst tvaryn vid zhorstokoho povodzhennia” vid 21.02.2006 r., No 3447 [The Law of Ukraine "On the Protection of animals from ill-treatment" of 02.21. 006, No. 3447]. [in Ukrainian].

Kozhemiakin, Yu.M., Khromova, O.S., & Filonen­ko, M.A. (2002). Naukovo-praktychni rekomendatsii z utrymannia laboratornykh tvaryn ta robota z nymy [Scientific and practical recommendations for the maintenance of laboratory animals and work with them]. Kyiv: Avitsena [in Ukrainian].

(1986). European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes. Council of Europe. Strasbourg.

Kamyshnykov, V.S. (2002). Spravochnyk po kliniko-byokhymycheskoy laboratornoy diagnostike [The manual on clinical and biochemical laboratory diagnostics]. Minsk. 1, 546-447 [in Russian].

Meshchyshen, I.F. (1998). Metod vyznachennia okysniuvalnoi modyfikatsii bilkiv plazmy (syrovatky) krovi [Method of determination of oxidizing modification of plasma proteins (serum)]. Bukovyn. med. visn. – Bukovyna Medical Bulletin, 1, 156-158 [in Ukrainian].

Bernard Rosner (2010). Fundamentals of Bio­statistics. Boston, USA.

Gutteridge, J.M., & Halliwell, B. (2010). Anti­oxidants: molecules, medicines and myths. Biochem. Biophys. Res. Commun., 393, 564.3.

Levin, G.Ya., & Egorikhina, M.N. (2008). Rol perikesnogo okisleniya lipidov v agregatsii kletok krovi pri ozhogovoy bolezni [Role of lipid peroxidation in aggregation of blood cells in case of burn disease]. Klin. lab. diagnostika – Clinical Laboratory Diagnostics, 8, 43-44 [in Russian].

Shin-Kyo Chung, Toshihiko Osawa, & Shunro Kawakishi (1997). Hydroxyl radical-scavenging effects of spices and scavengers from brown mustard (Brassicanigra). Biosei. Biotedl. Biochem., 61 (1), 118-123. Retrieved from: https://doi.org/10.1271/bbb.61.118.

Published
2019-07-11
How to Cite
Dmukhalska, Y. B., & Yaroshenko, T. Y. (2019). THE OXIDATION PROCESSES IN DIFFERENT AGE RATS UNDER THE CONDITIONS OF COMPLEX PERSISTENT EFFECT OF HEAVY METAL SALTS AND PHOSPHORORGANIC PESTICIDES . Medical and Clinical Chemistry, (2), 97-102. https://doi.org/10.11603/mcch.2410-681X.2019.v.i2.10301
Section
ORIGINAL INVESTIGATIONS