CHANGES IN THE CYTOKINE PROFILE IN RATS WITH PERIODONTITIS ON THE BACKGROUND OF HYPER- AND HYPOTHYROIDISM
Introduction. In Ukraine, the prevalence of periodontal disease among the population aged 35–44 years ranges from 92 % to 98 %. The question of the hormonal regulation of inflammatory reactions in the periodontium and the characteristics of their development against the background of thyroid dysfunction remain insufficiently studied.
The aim of the study – to investigate the cytokine profile of blood serum and periodontal homogenate in rats with periodontitis without comorbidities and against the background of hyper- and hypothyroidism.
Research Methods. The study was performed on white male rats, in which periodontitis, periodontitis on the background of hyperthyroidism and periodontitis on the background of hypothyroidism were modeled. The content of interleukins (IL) 1β, 4 and 10 in the blood serum and periodontal homogenate supernatant was determined by enzyme-linked immunosorbent assay using RayBio reagent kits manufactured by RayBiotech (USA).
Results and Discussion. The content of proinflammatory IL-1β in blood serum of rats with simulated periodontitis increased by 1.6 times (p<0.001) relative to the control group. In rats with simulated periodontitis, against the background of hyperthyroidism, this index increased by 2.5 times (p<0.001) relative to the control group. It should be noted that the concentration of IL-1β in the blood serum of hyperthyroidic rats by 51.5 % probably exceeded the value of this indicator under condition of periodontitis without concomitant pathology and by 37.7 % (p<0.001) – under the condition of periodontitis оn the background of hypothyroidism. The content of anti-inflammatory IL-10 decreased by 51.3 % (p<0.001) in blood serum of rats with simulated periodontium volume relative to the control group. In rats with simulated periodontitis оn the background of hyperthyroidism, this indicator decreased by 69.9 % (p<0.001) relative to the control group. At the same time, the content of IL-10 in the blood serum of hyperthyroidic rats by 38.1 % was significantly lower than the given indicator in the case of periodontitis without concomitant pathology and by 19.0 % (p<0.05) – under the condition of periodontitis against the background of hypothyroidism.
Conclusions. Experimental periodontitis is accompanied by a pronounced increase in the concentration of proinflammatory cytokines against the background of a decrease in the concentration of anti-inflammatory cytokines, both in the periodontal homogenate and in the blood serum, which indicates the development of not only local inflammatory reactions, but also systemic ones. An imbalance of thyroid hormones affects the course of inflammation in experimental periodontitis, especially in hyperthyroidism.
Frencken, J.E., Sharma, P., Stenhouse, L., Green, D., Laverty, D. & Dietrich, T. (2017). Global epidemiology of dental caries and severe periodontitis – a comprehensive review. J. Clin. Periodontol., 44 (18), 94-105.
Antonenko, M.Yu., Zelinska, N.A., Znachkova, O.A., Melnychuk, T.A. & Syroishko, M.V. (2016). Optymizatsiia peredoperatsiinoi pidhotovky v kompleksnomu likuvanni khvorykh na heneralizovanyi parodontyt [Optimization of preoperative preparation in the complex treatment of patients with generalized periodontitis]. Ukrainskyi zhurnal medytsyny, biolohii ta sportu – Ukrainian Journal of Medicine, Biology and Sports, 2 (2), 8-11 [in Ukrainian].
Srivastava, N., Nayak, P.A. & Rana S. (2017). Point of care – a novel approach to periodontal diagnosis – a review. J. Clin. Diagn. Res.,11 (8), ZE01-ZE06.
Krynytska, I.Ya. (2012). Rol aktyvnykh form kysniu u rozvytku hepatopulmonalnoho syndromu v eksperymenti [The role of reactive oxygen species in the development of hepatopulmonary syndrome in the experiment]. Zdobutky klinichnoi i eksperymentalnoi medytsyny – Achievements of Clinical and Experimental Medicine, 1, 72-76 [in Ukrainian].
Krynytska, I.Ya. (2013). Riven apoptychno ta nekrotychno zminenykh monotsytiv ta alveoliarnykh makrofahiv za umovy eksperymentalnoho hepatopulmonalnoho syndromu [Level of apoptotic and necrotizing altered monocytes and alveolar macrophages under experimental hepatopulmonary syndrome]. Svit medytsyny ta biolohii – World of Medicine and Biology, 2, 46-49 [in Ukrainian].
Almerich-Silla, J.M., Montiel-Company, J.M., Pastor, S., Serrano, F., Puig-Silla, M. & Dasi, F. (2015). Oxidative stress parameters in saliva and its association with periodontal disease and types of bacteria. Dis. Markers. Article ID 653537.
Shcherba, V., Vydoinyk, O., Posolenyk, L. & Korda, M. (2019). The influence of thyroid hormones on mitochondrial mechanisms of blood neutrophils’ apoptosis in case of experimental periodontitis. Archives of the Balkan Medical Union, 54, 1, 64-71.
Duvina, M. & Barbato, L. (2012). Biochemical markers as predictors of bone remodelling in dental disorders: a narrative description of literature. Clin. Cases Miner. Bone Metab., 9 (2), 100-106.
Moyseeva, E.G. (2008). Metabolicheskyy gomeostaz i imunnaya reaktivnost organizma v dinamyke vospaleniya v tkanyakh parodonta [Metabolic homeostasis and immune reactivity of the organism in the dynamics of inflammation in periodontal tissues]. Extended abstract of Doctor’s thesis. Sumy: SumSU [in Russian].
Ratushnenko, V.O. (2010). Funktsionalna rol tiol-dysulfidnoi systemy pry eksperymentalnomu hipo- i hipertyreozi [Functional role of thiol-disulphide system in experimental hypo- and hyperthyroidism]. Odeskyi medychnyi zhurnal – Odesa Medical Journal, 2 (118), 17-20 [in Ukrainian].
European convention for the protection of vertebrate animals used for experimental and other scientific purposes. (1986). Council of Europe. Strasbourg, 123, 52.
Avdieiev, O.V. (2013). Stupin aktyvnosti fosfataz pry eksperymentalnomu parodontyti ta za yoho korektsii [Stage of phosphatases activity during experimental parodontitis and after its correction]. Klinichna stomatolohiia – Clinical Stomatology, 3-4, 13-17 [in Ukrainian].
Turner, M.D., Nedjai, B. & Hurst, T. (2014). Cytokines and chemokines: At the crossroads of cell signalling and inflammatory disease. Biochim. Biophys. Acta. Mol. Cell Res., 1843, 11, 2563-2582.
Rawlison, A., Datali, M.H. & Rahman, S. (2000). Interleukin-1 and IL-1 receptor antagonist in gingival crevicular fluid. Clin. Periodontol., 27, 738-743.
Aleksandrov, E.I. (2014). Mikroflora i immunologicheskaya rezistentnost pri kariyese zubov i zabolevaniyakh parodonta na fone sakharnogo diabeta [Microflora and immunological resistance in case of dental caries and periodontal diseases on the background of diabetes mellitus]. Medyko-sotsialni problemy simi – Medico-social Problems of the Family, 19, 1, 109-114 [in Russian].
Ziablov, E.V., Chesnokova, N.P. & Barsukov, V.Yu. (2011). Zakonomernosti izmeneniy tsitokinovogo statusa u bolnykh follikulyarnoy i papilliarnoy formami raka shchitovidnoy zhelezy v dinamike rasprostraneniya neoplazii [Regularities of changes in cytokine status in patients with follicular and papillary forms of thyroid cancer in the dynamics of the spread of neoplasia]. Fundamentalnye issledovaniya – Basic Research, 5, 62-67 [in Russian].
Engebretson, S.P., Grbic, J.T., Singer, R. & Lamsret, I.B. (2002). GCF IL-1beta profiles in periodontal disease. J. Clin. Periodontol., 29, 48-53.
Tsarev, V.N. & Nikolayeva, E.N. (2007). Allelnyy polimorfizm genov IL-1α i IL-1β u bolnykh s khronicheskimi vospalitelnymi zabolevaniyami parodonta [Allelic polymorphism of the genes IL-1α and IL-1β in patients with chronic inflammatory periodontal diseases]. Vestnik RAMN – Bulletin of the Russian Academy of Medical Sciences, 3, 43-47 [in Russian].
Mendonça, M.S., Peraçolli, T.S., Silva-Vergara, M.L., Ribeiro, S.C., Oliveira, R.F., Mendes, R.P. & Rodrigues, V.Jr. (2015). High interleukin-4 expression and interleukin-4 gene polymorphisms are associated with susceptibility to human paracoccidioidomycosis. Jr. Mem. Inst. Oswaldo Cruz.,110, 6, 781-785.
Dutzan, N., Gamonal, J., Silva, A., Sanz, M. & Vernal, R. (2009). Over-expression of fork-head box P3 and its association with receptor activator of nuclear factor-kappa B ligand, interleukin (IL) – 17, IL – 10 and transforming growth factor-beta during the progression of chronic periodontitis. J. Clin. Periodontal., 36, 396-403.
Sabirova, A.I. (2016). Tsitokinovyy status u bolnykh generalizovannym parodontitom i metabolicheskim sindromom [Cytokine status in patients with generalized periodontitis and metabolic syndrome]. Vestnik KRSU – Bulletin of KRSU, 16, 7, 102-105 [in Russian].